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Identification of the main quinolone resistance determinant in Campylobacter jejuni and Campylobacter coli by MAMA-DEG PCR

2016-03Journal article Restricted access
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datacite.subject.sdg12:Produção e Consumo Sustentáveis
datacite.subject.sdg13:Ação Climática
datacite.subject.sdg14:Proteger a Vida Marinha
dc.contributor.authorHormeño, Lorena
dc.contributor.authorPalomo, Gonzalo
dc.contributor.authorUgarte-Ruiz, María
dc.contributor.authorPorrero, M. Concepción
dc.contributor.authorBorge, Carmen
dc.contributor.authorVadillo, Santiago
dc.contributor.authorPíriz, Segundo
dc.contributor.authorDomínguez, Lucas
dc.contributor.authorCampos, Maria Jorge
dc.contributor.authorQuesada, Alberto
dc.date.accessioned2025-07-15T10:53:12Z
dc.date.available2025-07-15T10:53:12Z
dc.date.issued2016-03
dc.description.abstractAmong zoonotic diseases, campylobacteriosis stands out as the major bacterial infection producing human gastroenteritis. Antimicrobial therapy, only recommended in critical cases, is challenged by resistance mechanisms that should be unambiguously detected for achievement of effective treatments. Quinolone (ciprofloxacin) resistance of Campylobacter jejuni and Campylobacter coli, the2main Campylobacter detected in humans, is conferred by the mutation gyrA C-257-T, which can be genotyped by several methods that require a previous identification of the pathogen species to circumvent the sequence polymorphism of the gene. A multiplex PCR, based on degenerated oligonucleotides, has been designed for unambiguous identification of the quinolone resistance determinant in Campylobacter spp. isolates. The method was verified with 249 Campylobacter strains isolated from humans (141 isolates) and from the 3 most important animal sources for this zoonosis: poultry (34 isolates), swine (38 isolates), and cattle (36 isolates). High resistance to ciprofloxacin, MIC above 4 μg/mL, linked to the mutated genotype predicted by MAMA-DEG PCR (mismatch amplification mutation assay PCR with degenerated primers) was found frequently among isolates from the different hosts.eng
dc.description.sponsorshipThe authors wish to thank for their support the Ministry of Innovation, Science and Technology of Spain (AGL2012-39028-C03-01 and AGL2012-39028-C03-03), the Department of Employ, Enterprise and Innovation of the regional government of Extremadura, Spain (Group CTS001), the University of Extremadura (Group MIVET), the Spanish Ministry of Agriculture, and the Autonomous Community of Madrid (S2009/AGR-1489 and S2013/ABI-2747).
dc.identifier.citationLorena Hormeño, Gonzalo Palomo, María Ugarte-Ruiz, M. Concepción Porrero, Carmen Borge, Santiago Vadillo, Segundo Píriz, Lucas Domínguez, Maria J. Campos, Alberto Quesada, Identification of the main quinolone resistance determinant in Campylobacter jejuni and Campylobacter coli by MAMA-DEG PCR, Diagnostic Microbiology and Infectious Disease, Volume 84, Issue 3, 2016, Pages 236-239, ISSN 0732-8893, https://doi.org/10.1016/j.diagmicrobio.2015.11.002
dc.identifier.doi10.1016/j.diagmicrobio.2015.11.002
dc.identifier.issn0732-8893
dc.identifier.urihttp://hdl.handle.net/10400.8/13640
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier BV
dc.relation.hasversionhttps://www.sciencedirect.com/science/article/pii/S0732889315004010?via%3Dihub
dc.relation.ispartofDiagnostic Microbiology and Infectious Disease
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectMAMA PCR
dc.subjectDegenerated primers
dc.subjectgyrA
dc.subjectFluoroquinolones
dc.subjectCiprofloxacin-resistance
dc.subjectCampylobacter jejuni
dc.subjectCampylobacter coli
dc.titleIdentification of the main quinolone resistance determinant in Campylobacter jejuni and Campylobacter coli by MAMA-DEG PCReng
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage239
oaire.citation.issue3
oaire.citation.startPage236
oaire.citation.titleDiagnostic Microbiology and Infectious Disease
oaire.citation.volume84
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameCampos
person.givenNameMaria Jorge
person.identifier174191
person.identifier.ciencia-id2416-7867-2D40
person.identifier.orcid0000-0002-0883-0610
person.identifier.ridE-5591-2014
person.identifier.scopus-author-id55767069800
relation.isAuthorOfPublication6d1bf3db-fdba-4bea-b65d-94491f841659
relation.isAuthorOfPublication.latestForDiscovery6d1bf3db-fdba-4bea-b65d-94491f841659

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