Logo do repositório
 
Publicação

Adhesion, proliferation and distribution of human mesenchymal stem/stromal cells (MSCs) in Poly(ɛ -caprolactone) (PCL) scaffolds with different pore sizes

2013-10Comunicação em conferência Acesso aberto
Ver registo simples
datacite.subject.fosEngenharia e Tecnologia
datacite.subject.sdg09:Indústria, Inovação e Infraestruturas
datacite.subject.sdg12:Produção e Consumo Sustentáveis
dc.contributor.authorMoura, C. S.
dc.contributor.authorBiscaia, S.
dc.contributor.authorViana, T.
dc.contributor.authorBártolo, P. J.
dc.contributor.authorSilva, C. L. da
dc.contributor.authorCabral, J. S.
dc.contributor.authorFerreira, F. C.
dc.date.accessioned2026-06-24T11:38:27Z
dc.date.available2026-06-24T11:38:27Z
dc.date.issued2013-10
dc.descriptionConference date - 1 October 2013 - 5 October 2013; Conference code - 101946
dc.descriptionLink para o documento - https://www.google.com/search?q=Adhesion%2C+proliferation+and+distribution+of+human+mesenchymal+stemstromal+cells+%28MSCs%29+in+Poly%28%CE%B5-caprolactone%29+%28PCL%29+scaffolds+with+different+pore+sizes&rlz=1C1GCEU_pt-PTPT1117PT1117&oq=Adhesion%2C+proliferation+and+distribution+of+human+mesenchymal+stemstromal+cells+%28MSCs%29+in+Poly%28%CE%B5-caprolactone%29+%28PCL%29+scaffolds+with+different+pore+sizes&gs_lcrp=EgZjaHJvbWUyBggAEEUYOdIBCTE4OTdqMGoxNagCCLACAfEFpPwT3wgDYK0&sourceid=chrome&ie=UTF-8
dc.description.abstractTissue engineering, combining the use of biomaterials, mesenchymal stem/stromal cells (MSCs) and optimized culture medium formulations, is a very promising research field for tissue regeneration. This work is focused on the application of Poly(ε-caprolactone) scaffolds designed to have a spacing gradient between orthogonal fibres with diameters of 0.3mm with pore sizes ranging from 190 to 390 μm. Human bone marrow (BM)-derived MSCs, were used to evaluate cell adhesion, proliferation and distribution within the scaffolds. Specifically, we hypothesized if seeding MSC in scaffold regions with different pore sizes would influence cell proliferation. Therefore, MSC were seeded at the centre of scaffolds with either larger (390 μm) or smaller pores (190 μm). Results obtained showed similar levels of adhesion and proliferation in both configurations, with fold increase in total cell number of 5 after 13 days of cultivation; which indicates that no limitation for cell proliferation within the range of pore sizes studied is observed.eng
dc.description.sponsorshipThis research is sponsored by the Portuguese Foundation for Science and Technology through SFRH/BD/73970/2010, IF/00442/2012, MIT Portugal Program, Bioengineering Systems Focus Area and through the strategic project Pest-OE/EME/UI4044/2011.
dc.identifier.citationMoura, C. S., Biscaia, S., Viana, T., Bártolo, P. J., Da Silva, C. L., Cabral, J. S., & Ferreira, F. C. (2014). Adhesion, proliferation and distribution of human mesenchymal stem/stromal cells (MSCs) in Poly (ε-caprolactone)(PCL) scaffolds with different pore sizes. In 6th International Conference on Advanced Research in Virtual and Physical Prototyping, VR@ P 2013.
dc.identifier.doi10.1201/b15961-36
dc.identifier.isbn9781138001374
dc.identifier.isbn9781315817415
dc.identifier.urihttp://hdl.handle.net/10400.8/16451
dc.language.isoeng
dc.peerreviewedyes
dc.publisherTaylor and Francis
dc.relationDESIGN AND FABRICATION OF ZONAL CARTILAGE CONSTRUCTS
dc.relationStrategic Project - UI 4044 - 2011-2012
dc.relation.hasversionhttps://scholar.google.com/scholar?q=Adhesion%2C%20proliferation%20and%20distribution%20of%20human%20mesenchymal%20stem%2Fstromal%20cells%20%28MSCs%29%20in%20Poly%28%CE%B5-caprolactone%29%20%28PCL%29%20scaffolds%20with%20different%20pore%20sizes
dc.relation.ispartofHigh Value Manufacturing: Advanced Research in Virtual and Rapid Prototyping
dc.rights.uriN/A
dc.subjectBiological materials
dc.subjectCell adhesion
dc.subjectCell culture
dc.subjectCell proliferation
dc.subjectFlowcharting
dc.subjectIndustrial research
dc.subjectPore size
dc.subjectRapid prototyping
dc.titleAdhesion, proliferation and distribution of human mesenchymal stem/stromal cells (MSCs) in Poly(ɛ -caprolactone) (PCL) scaffolds with different pore sizeseng
dc.typeconference paper
dspace.entity.typePublication
oaire.awardNumberSFRH/BD/73970/2010
oaire.awardNumberPEst-OE/EME/UI4044/2011
oaire.awardTitleDESIGN AND FABRICATION OF ZONAL CARTILAGE CONSTRUCTS
oaire.awardTitleStrategic Project - UI 4044 - 2011-2012
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F73970%2F2010/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/PEst-OE%2FEME%2FUI4044%2F2011/PT
oaire.citation.conferenceDate2013-10
oaire.citation.conferencePlaceLeiria, Portugal
oaire.citation.titleHigh Value Manufacturing: Advanced Research in Virtual and Rapid Prototyping - Proceedings of the 6th International Conference on Advanced Research and Rapid Prototyping, VR@P 2013
oaire.fundingStream6817 - DCRRNI ID
oaire.versionhttp://purl.org/coar/version/c_ab4af688f83e57aa
person.familyNameMonteiro de Moura
person.familyNameBiscaia
person.familyNameViana
person.familyNameBartolo
person.givenNameCarla Sofia
person.givenNameSara
person.givenNameTânia
person.givenNamePaulo
person.identifier203086
person.identifier.ciencia-id671C-8FEC-A14A
person.identifier.ciencia-id0516-3C03-93F3
person.identifier.ciencia-idB41D-8BE0-7C9D
person.identifier.ciencia-id5810-9BF9-4522
person.identifier.orcid0000-0003-2610-1005
person.identifier.orcid0000-0002-2609-6878
person.identifier.orcid0000-0002-9688-647X
person.identifier.orcid0000-0003-3683-726X
person.identifier.ridO-1146-2013
person.identifier.ridO-1147-2013
person.identifier.ridF-2421-2013
person.identifier.scopus-author-id55867382400
person.identifier.scopus-author-id55885892100
person.identifier.scopus-author-id6603353041
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
relation.isAuthorOfPublication33d084bb-ac8d-454a-ac58-25be1331ce04
relation.isAuthorOfPublicationa5f3b2b2-264b-42d3-9a94-c27e1e0cecf5
relation.isAuthorOfPublicationfe6d67b5-016d-4953-a5eb-9e357af79793
relation.isAuthorOfPublicationab44d1ae-46d0-45c2-b19f-200024b5a990
relation.isAuthorOfPublication.latestForDiscovery33d084bb-ac8d-454a-ac58-25be1331ce04
relation.isProjectOfPublicationcdbe26aa-314b-4fc8-9f76-f3fda8820f2f
relation.isProjectOfPublication44bd1c93-9ee1-4685-a408-c2baa61ffbcd
relation.isProjectOfPublication.latestForDiscoverycdbe26aa-314b-4fc8-9f76-f3fda8820f2f

Ficheiros

Principais
A mostrar 1 - 1 de 1
Miniatura
Nome:
Adhesion, proliferation and distribution of human mesenchymal stemstromal cells (MSCs) in Poly(ε-caprolactone) (PCL) scaffolds with different pore sizes.pdf
Tamanho:
281.73 KB
Formato:
Adobe Portable Document Format
Descrição:
Tissue engineering, combining the use of biomaterials, mesenchymal stem/stromal cells (MSCs) and optimized culture medium formulations, is a very promising research field for tissue regeneration. This work is focused on the application of Poly(ε-caprolactone) scaffolds designed to have a spacing gradient between orthogonal fibres with diameters of 0.3mm with pore sizes ranging from 190 to 390 μm. Human bone marrow (BM)-derived MSCs, were used to evaluate cell adhesion, proliferation and distribution within the scaffolds. Specifically, we hypothesized if seeding MSC in scaffold regions with different pore sizes would influence cell proliferation. Therefore, MSC were seeded at the centre of scaffolds with either larger (390 μm) or smaller pores (190 μm). Results obtained showed similar levels of adhesion and proliferation in both configurations, with fold increase in total cell number of 5 after 13 days of cultivation; which indicates that no limitation for cell proliferation within the range of pore sizes studied is observed.
Ver/Abrir
Licença
A mostrar 1 - 1 de 1
Miniatura indisponível
Nome:
license.txt
Tamanho:
1.32 KB
Formato:
Item-specific license agreed upon to submission
Descrição:
Ver/Abrir

Coleções

CDRsp - Comunicações em conferências internacionais
ESTG - Comunicações em conferências e congressos internacionais