Dilemma on plasmid DNA purification: binding capacity vs selectivity

Valente, J. F. A.; Queiroz, J. A.; Sousa, F.

http://hdl.handle.net/10400.8/8223

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Autores

Valente, J. F. A.

Queiroz, J. A.

Sousa, F.

Resumo(s)

Plasmid DNA chromatography is a powerful field in constant development and evolution. The use of this technique is considered mandatory in the production of an efficient and safe formulation to be applied for plasmid-mediated gene therapy. Concerning this, the search for an ideal chromatographic support/ligand combination motivated scientist to pursue a continuous improvement on the plasmid chromatography performance, looking for a progression on the ligands and supports used. The present review explores the different approaches used over time to purify plasmid DNA, ambitioning both high recovery and high purity levels. Overall, it is presented a critical discussion relying on the relevance of the binding capacity versus selectivity of the supports.

Palavras-chave

Specific ligands Selectivity DNA chromatography Chromatographic supports Binding capacity

URI

http://hdl.handle.net/10400.8/8223

Citação

J.F.A. Valente, J.A. Queiroz, F. Sousa, Dilemma on plasmid DNA purification: binding capacity vs selectivity, Journal of Chromatography A, Volume 1637, 2021, 461848, ISSN 0021-9673, https://doi.org/10.1016/j.chroma.2020.461848.

Editora

Elsevier

DOI

10.1016/j.chroma.2020.461848

Coleções

CDRsp - Artigos em revistas internacionais

Licença CC

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