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Carica papaya latex: A low‐cost biocatalyst for human milk fat substitutes production

2012-01-12Artigo científico Acesso restrito
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datacite.subject.fosCiências Naturais::Ciências Biológicas
datacite.subject.sdg12:Produção e Consumo Sustentáveis
datacite.subject.sdg13:Ação Climática
datacite.subject.sdg14:Proteger a Vida Marinha
dc.contributor.authorTecelão, Carla
dc.contributor.authorRivera, Ivanna
dc.contributor.authorSandoval, Georgina
dc.contributor.authorFerreira‐Dias, Suzana
dc.date.accessioned2026-01-13T15:47:38Z
dc.date.available2026-01-13T15:47:38Z
dc.date.issued2012-01-12
dc.description.abstract<jats:title>Abstract</jats:title><jats:p>This work aims at evaluating the potential of <jats:italic>Carica papaya</jats:italic> lipase (CPL) self‐immobilized in papaya latex as a biocatalyst for the synthesis of human milk fat substitutes (HMFS), to be used as a low‐cost alternative to commercial lipases. Two different CPL preparations, one extracted from the papaya fruit (CPL I) and the other from petiole leaves (CPL II) of papaya tree, were tested as catalysts for the acidolysis between tripalmitin and (i) oleic acid or (ii) omega‐3 PUFA, batchwise, at 60°C, in solvent‐free media. After 24 h, molar incorporation was higher for oleic acid (22.1 mol%) when CPL I was used. This biocatalyst was selected for further studies. RSM was used to model reaction conditions: medium formulation (molar ratio oleic acid/tripalmitin, MR, 1.2:1–6.8:1) and temperature (58–72°C). Acyl migration decreased with MR increase. In batch operational stability assays at 60°C, using MR of 2:1 and 6:1, the highest stability was observed for a MR of 2:1.</jats:p><jats:p><jats:bold>Practical applications:</jats:bold> The use of this biocatalyst is a feasible way to valorize papaya agro‐residues which represent an important environmental problem in the producing countries. The obtained results were rather promising since, with this almost zero‐cost biocatalyst, it was possible to produce a high added‐value product (HMFS). Under optimized conditions, the obtained results were comparable with those obtained with expensive immobilized commercial lipases.</jats:p>
dc.description.sponsorshipThe authors are grateful to EPAX, AS, Norway, for the gift of the EPAX 1050TG and to the Fundação para a Ciência e a Tecnologia (FCT), Portugal, for a PhD fellowship for Mrs. Carla Tecelão (SFRH/BD/45773/2008). Studies on preparation of CPL I and II were supported by CONACYT (Mexico) grant CB-2008-104429 and ECOS (France) – ANUIES/CONACYT (Mexico) grant M08-A03. The petiole wastes used in the preparation of CPL II were kindly provided by Dr. Juan Carlos Mateos (CIATEJ, Mexico). This study was also supported by the Program CYTED, under the scope of the Ibero-American Network for the Extraction and Enzymatic Transformation of Functional Ingredients and Nutraceuticals from Regional Plants and Agro-Residues (ENZNUT).
dc.identifier.citationTecelao, C., Rivera, I., Sandoval, G., & Ferreira‐Dias, S. (2012). Carica papaya latex: A low‐cost biocatalyst for human milk fat substitutes production. European journal of lipid science and technology, 114(3), 266-276.
dc.identifier.doi10.1002/ejlt.201100226
dc.identifier.issn1438-7697
dc.identifier.issn1438-9312
dc.identifier.urihttp://hdl.handle.net/10400.8/15319
dc.language.isoeng
dc.peerreviewedyes
dc.publisherWiley
dc.relation.hasversionhttps://onlinelibrary.wiley.com/doi/full/10.1002/ejlt.201100226
dc.relation.ispartofEuropean Journal of Lipid Science and Technology
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectAcidolysis
dc.subjectCarica papaya wastes
dc.subjectHuman milk fat substitutes
dc.subjectLatex
dc.subjectLipase
dc.titleCarica papaya latex: A low‐cost biocatalyst for human milk fat substitutes productioneng
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage276
oaire.citation.issue3
oaire.citation.startPage266
oaire.citation.titleEuropean Journal of Lipid Science and Technology
oaire.citation.volume114
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameTecelão
person.givenNameCarla
person.identifier.ciencia-id9B1D-4AC0-1B03
person.identifier.orcid0000-0003-2423-0495
person.identifier.scopus-author-id7801371939
relation.isAuthorOfPublication0d890808-4e69-4e6c-a631-802f1a7dce1f
relation.isAuthorOfPublication.latestForDiscovery0d890808-4e69-4e6c-a631-802f1a7dce1f

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