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Obesogens beyond Vertebrates: Lipid Perturbation by Tributyltin in the Crustacean Daphnia magna.

2015-08Journal article Open access
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datacite.subject.fosCiências Naturais::Ciências Biológicas
datacite.subject.sdg14:Proteger a Vida Marinha
dc.contributor.authorJordão, Rita
dc.contributor.authorCasas, Josefina
dc.contributor.authorFabrias, Gemma
dc.contributor.authorCampos, Bruno
dc.contributor.authorPiña, Benjamín
dc.contributor.authorLemos, Marco
dc.contributor.authorSoares, Amadeu M V M
dc.contributor.authorTauler, Romà
dc.contributor.authorBarata, Carlos
dc.date.accessioned2025-06-21T11:19:40Z
dc.date.available2025-06-21T11:19:40Z
dc.date.issued2015-08
dc.description
dc.description.abstractThe analysis of obesogenic effects in invertebrates is limited by our poor knowledge of the regulatory pathways of lipid metabolism. Recent data from the crustacean Daphnia magna points to three signaling hormonal pathways related to the molting and reproductive cycles [retinoic X receptor (RXR), juvenile hormone (JH), and ecdysone] as putative targets for exogenous obesogens. Objective: The present study addresses the disruptive effects of the model obesogen tributyltin (TBT) on the lipid homeostasis in Daphnia during the molting and reproductive cycle, its genetic control, and health consequences of its disruption. Methods: D. magna individuals were exposed to low and high levels of TBT. Reproductive effects were assessed by Life History analysis methods. Quantitative and qualitative changes in lipid droplets during molting and the reproductive cycle were studied using Nile red staining. Lipid composition and dynamics were analyzed by ultra-performance liquid chromatography coupled to a time-of-flight mass spectrometer. Relative abundances of mRNA from different genes related to RXR, ecdysone, and JH signaling pathways were studied by qRT-PCR. Results and Conclusions: TBT disrupted the dynamics of neutral lipids, impairing the transfer of triacylglycerols to eggs and hence promoting their accumulation in adult individuals. TBT’s disruptive effects translated into a lower fitness for offspring and adults. Co-regulation of gene transcripts suggests that TBT activates the ecdysone, JH, and RXR receptor signaling pathways, presumably through the already proposed interaction with RXR. These findings indicate the presence of obesogenic effects in a nonvertebrate species. © 2015, Public Health Services, US Dept of Health and Human Services. All rights reserved.eng
dc.description.sponsorshipThis project was supported by the Spanish Ministry of Education and Science (MEC CTM2011-30471-C02-01 and by Advance grant–EU (European Union) grant ERC-2012-AdG-320737. The Portuguese Foundation for Science and Technology (FCT) supported the doctoral fellowship of R.J. (SFRH/BD/79453/2011).
dc.identifier.citationJordão R, Casas J, Fabrias G, Campos B, Piña B, Lemos MF, Soares AM, Tauler R, Barata C. Obesogens beyond Vertebrates: Lipid Perturbation by Tributyltin in the Crustacean Daphnia magna. Environ Health Perspect. 2015 Aug;123(8):813-9. doi: 10.1289/ehp.1409163. Epub 2015 Mar 24. PMID: 25802986; PMCID: PMC4529017.
dc.identifier.doi10.1289/ehp.1409163
dc.identifier.issn00916765
dc.identifier.other25802986
dc.identifier.urihttp://hdl.handle.net/10400.8/13362
dc.language.isoeng
dc.peerreviewedyes
dc.publisherPublic Health Services, US Dept of Health and Human Services
dc.relationHOLOTOX - INTEGRATIVE APPROACH TO CHARACTERIZE NEW MECHANISMS OF TOXICITY OF POLLUTANTS IN AQUATIC INVERTEBRATES
dc.relation.hasversionhttps://pmc.ncbi.nlm.nih.gov/articles/PMC4529017/
dc.rights.uriN/A
dc.subjectvertebrates
dc.titleObesogens beyond Vertebrates: Lipid Perturbation by Tributyltin in the Crustacean Daphnia magna.eng
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleHOLOTOX - INTEGRATIVE APPROACH TO CHARACTERIZE NEW MECHANISMS OF TOXICITY OF POLLUTANTS IN AQUATIC INVERTEBRATES
oaire.awardURIhttp://hdl.handle.net/10400.8/13361
oaire.citation.endPage819
oaire.citation.issue8
oaire.citation.startPage813
oaire.citation.titleEnvironmental Health Perspectives
oaire.citation.volume123
oaire.fundingStreamFARH
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameLemos
person.givenNameMarco
person.identifier996337
person.identifier.orcid0000-0001-9887-1864
person.identifier.ridF-7951-2011
person.identifier.scopus-author-id7006042884
relation.isAuthorOfPublicationf21e5540-df76-43e9-ad64-93edd70da1f1
relation.isAuthorOfPublication.latestForDiscoveryf21e5540-df76-43e9-ad64-93edd70da1f1
relation.isProjectOfPublication23982502-d9de-4162-9d8f-0fde4e51f135
relation.isProjectOfPublication.latestForDiscovery23982502-d9de-4162-9d8f-0fde4e51f135

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