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Selective Inhibition of Clade A Phosphatases Type 2C by PYR/PYL/RCAR Abscisic Acid Receptors

2011-12-23Artigo científico Acesso aberto
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datacite.subject.fosCiências Naturais::Ciências Biológicas
datacite.subject.sdg02:Erradicar a Fome
datacite.subject.sdg08:Trabalho Digno e Crescimento Económico
datacite.subject.sdg15:Proteger a Vida Terrestre
dc.contributor.authorAntoni, Regina
dc.contributor.authorGonzalez-Guzman, Miguel
dc.contributor.authorRodriguez, Lesia
dc.contributor.authorRodrigues, Américo
dc.contributor.authorPizzio, Gaston A.
dc.contributor.authorRodriguez, Pedro L.
dc.date.accessioned2026-02-18T18:47:49Z
dc.date.available2026-02-18T18:47:49Z
dc.date.issued2011-12-23
dc.description.abstract<jats:title>Abstract</jats:title> <jats:p>Clade A protein phosphatases type 2C (PP2Cs) are negative regulators of abscisic acid (ABA) signaling that are inhibited in an ABA-dependent manner by PYRABACTIN RESISTANCE1 (PYR1)/PYR1-LIKE (PYL)/REGULATORY COMPONENTS OF ABA RECEPTORS (RCAR) intracellular receptors. We provide genetic evidence that a previously uncharacterized member of this PP2C family in Arabidopsis (Arabidopsis thaliana), At5g59220, is a negative regulator of osmotic stress and ABA signaling and that this function was only apparent when double loss-of-function mutants with pp2ca-1/ahg3 were generated. At5g59220-green fluorescent protein and its close relative PP2CA-green fluorescent protein showed a predominant nuclear localization; however, hemagglutinin-tagged versions were also localized to cytosol and microsomal pellets. At5g59220 was selectively inhibited by some PYR/PYL ABA receptors, and close relatives of this PP2C, such as PP2CA/ABA-HYPERSENSITIVE GERMINATION3 (AHG3) and AHG1, showed a contrasting sensitivity to PYR/PYL inhibition. Interestingly, AHG1 was resistant to inhibition by the PYR/PYL receptors tested, which suggests that this seed-specific phosphatase is still able to regulate ABA signaling in the presence of ABA and PYR/PYL receptors and therefore to control the highly active ABA signaling pathway that operates during seed development. Moreover, the differential sensitivity of the phosphatases At5g59220 and PP2CA to inhibition by ABA receptors reveals a functional specialization of PYR/PYL ABA receptors to preferentially inhibit certain PP2Cs.</jats:p>eng
dc.description.sponsorshipThis work was supported by the Ministerio de Ciencia e Innovacion, Fondo Europeo de Desarrollo Regional, and Consejo Superior de Investigaciones Cientificas (grant nos. BIO2008–00221 and BIO2011–23446 to P.L.R; fellowships to R.A. and L.R.; Juan de la Cierva contract to M.G.-G.).
dc.identifier.citationRegina Antoni, Miguel Gonzalez-Guzman, Lesia Rodriguez, Americo Rodrigues, Gaston A. Pizzio, Pedro L. Rodriguez, Selective Inhibition of Clade A Phosphatases Type 2C by PYR/PYL/RCAR Abscisic Acid Receptors , Plant Physiology, Volume 158, Issue 2, February 2012, Pages 970–980, https://doi.org/10.1104/pp.111.188623
dc.identifier.doi10.1104/pp.111.188623
dc.identifier.issn1532-2548
dc.identifier.urihttp://hdl.handle.net/10400.8/15681
dc.language.isoeng
dc.peerreviewedyes
dc.publisherOxford University Press (OUP)
dc.relation.hasversionhttps://academic.oup.com/plphys/article-abstract/158/2/970/6109263?redirectedFrom=fulltext
dc.relation.ispartofPlant Physiology
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.titleSelective Inhibition of Clade A Phosphatases Type 2C by PYR/PYL/RCAR Abscisic Acid Receptorseng
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage980
oaire.citation.issue2
oaire.citation.startPage970
oaire.citation.titlePlant Physiology
oaire.citation.volume158
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameRodrigues
person.givenNameAmérico
person.identifier.orcid0000-0002-5293-5583
person.identifier.ridF-9573-2010
person.identifier.scopus-author-id15745701600
relation.isAuthorOfPublication718ad6e8-1d3a-4ce3-9870-e4ed6732c30b
relation.isAuthorOfPublication.latestForDiscovery718ad6e8-1d3a-4ce3-9870-e4ed6732c30b

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