Loading...
Publication
Desenvolvimento e aplicação de um pipeline analítico a novas dietas formuladas com microalgas para aplicação em aquacultura
| Name: | Description: | Size: | Format: | |
|---|---|---|---|---|
| 2.36 MB | Adobe PDF |
Authors
Abstract(s)
A atual expansão do setor de aquacultura está a impulsionar o desenvolvimento de produtos baseados em microalgas produzidas industrialmente. A implementação de dietas comerciais em maternidades de aquacultura, permite a redução da produção in loco de microalgas, contribuindo para a otimização do esforço de manutenção e redução dos custos de produção nas maternidades. A combinação de espécies de microalgas adaptadas aos requerimentos nutricionais das espécies alvo e adaptadas a diferentes sistemas de cultivo, é uma estratégia pouco explorada assim como, a utilização de aditivos nas formulações, tais como probióticos e quelantes de amónia.
Em larvicultura de peixes marinhos, as microalgas são utilizadas em 3 técnicas fundamentais: 1) “técnica de água verde”, 2) cultivo de rotíferos e 3) enriquecimento de alimento vivo. Desta forma, desenvolveram-se produtos adaptados às diferentes etapas de produção, nomeadamente, o produto piloto de tipologia pó, designado H2O Verde com Nannochloropsis sp e Chlorella sp. (CN) e com probióticos (CNP) para a “técnica de água verde”, de forma a aumentar a performance biológica das larvas de peixe marinhas. Para o cultivo de rotíferos, desenvolveram-se 3 produtos distintos para os diferentes modos e sistemas de produção. A formulação P.Rot Contínuo de tipologia pó, desenvolvida para o cultivo de rotíferos de forma semi-contínua, combina microalgas, probióticos e quelante de amónia para manutenção da qualidade da água. Em tipologia de concentrado líquido, desenvolveu-se o produto premium P. Rot Batch (18 % peso seco - PS) para cultivo de rotíferos em sistemas fechados e modo de produção batch. Constituído por uma solução diluente hipersalina modificada, permitiu a otimização da qualidade microbiológica do produto e do cultivo, aumentando a eficiência de produção. Os Recirculating aquaculture system’s (P. Rot RAS), começaram recentemente a ser utilizados para a produção intensiva de rotíferos em modo de produção semi-contínuo. Devido ao seu complexo sistema de filtragem, desenvolveu-se o produto P. Rot RAS, em concentrado líquido, com uma densidade mais reduzida (15 % PS) e com uma solução salina otimizada (35 ppt). Esta formulação permitiu a aplicação em sistemas RotiRAS® (IDEE aquaculture, França) sem colmatação de filtros e promoveu a eficiência do cultivo de rotíferos.
Atualmente, há uma grande procura por soluções alimentares para bivalves com microalgas produzidas industrialmente uma vez que, o setor se encontra em expansão devido à súbita necessidade de aumentar a produção de sementes (pós-larvas) de bivalves em maternidades, uma vez que as alterações climáticas promoveram o declínio abrupto do recrutamento natural. As dietas comerciais existentes, não respeitam as espécies e proporções adequadas para a nutrição dos bivalves. Desta forma, as dietas BIV 1 e BIV 2 foram desenvolvidas em concentrado líquido de baixa densidade (8 % PS), para o cultivo de pós-larvas e juvenis. Posteriormente, a dieta BIV 1 foi otimizada em termos de solução salina diluente.
Através do presente estágio foi possível desenvolver 7 produtos piloto para aquacultura através dos quais se estabeleceu um pipeline analítico de desenvolvimento de novos produtos de microalgas para aquacultura.
The current expansion of the aquaculture sector is driving the development of products based on industrially produced microalgae. The implementation of commercial diets in aquaculture hatcheries, allows the reduction of on-site production of microalgae, contributing to the optimization of maintenance efforts and reduction of production costs in the hatcheries. The combination of microalgae species adapted to the nutritional requirements of the target species and adapted to different culture systems is a little explored strategy, as well as the use of additives in the formulations, such as probiotics and ammonia chelators. In fish marine larviculture, microalgae are used in 3 fundamental techniques: 1) "green water technique", 2) rotifer culture and 3) live food enrichment. Therefore, products adapted to the different production stages were developed, namely the pilot product of the powder type, named H2O Verde with Nannochloropsis sp and Chlorella sp. (CN) and with probiotics (CNP) for the "green water technique", in order to increase the biological performance of marine fish larvae. For rotifer culture, 3 different products were developed for the different production modes and systems. The powder formulation P.Rot contínuo, developed for semi-continuous rotifer cultivation, combines microalgae, probiotics and ammonia chelator for maintenance of water quality. In liquid concentrate typology, the premium product P. Rot Batch (18% dry weight - PS) was developed for rotifer culture in closed systems and batch production mode. Consisting of a modified hypersaline diluent solution, it allowed the optimization of the microbiological quality of the product and the culture, increasing the production efficiency. Recirculating aquaculture system's (P. Rot RAS) have recently started to be used for intensive rotifer production in semi-continuous production mode. Due to its complex filtration system, the product P. Rot RAS formula was developed, in liquid concentrate, with a lower density (15% PS) and an optimized salt solution (35 ppt). This formulation allowed the application in RotiRAS® systems (IDEE aquaculture, France) without filter clogging and promoted rotifer culture efficiency. Currently, there is a high demand for industrially produced microalgae feed solutions for bivalves as the industry is expanding due to the sudden need to increase the production of bivalve seed (post-larvae) in hatcheries, as climate change has promoted a sharp decline in natural recruitment. Existing commercial diets do not respect the species and proportions appropriate for the nutrition of bivalves. Therefore, the BIV 1 and BIV 2 diets were developed in low density liquid concentrate (8 % PS), for the rearing of post-larvae and juveniles and juveniles, respectively. Subsequently, the BIV 1 diet was optimized in terms of diluent salt solution. Through this internship, it was possible to develop 7 pilot products for aquaculture through which, analytical pipeline for the development of new microalgae products for aquaculture was established.
The current expansion of the aquaculture sector is driving the development of products based on industrially produced microalgae. The implementation of commercial diets in aquaculture hatcheries, allows the reduction of on-site production of microalgae, contributing to the optimization of maintenance efforts and reduction of production costs in the hatcheries. The combination of microalgae species adapted to the nutritional requirements of the target species and adapted to different culture systems is a little explored strategy, as well as the use of additives in the formulations, such as probiotics and ammonia chelators. In fish marine larviculture, microalgae are used in 3 fundamental techniques: 1) "green water technique", 2) rotifer culture and 3) live food enrichment. Therefore, products adapted to the different production stages were developed, namely the pilot product of the powder type, named H2O Verde with Nannochloropsis sp and Chlorella sp. (CN) and with probiotics (CNP) for the "green water technique", in order to increase the biological performance of marine fish larvae. For rotifer culture, 3 different products were developed for the different production modes and systems. The powder formulation P.Rot contínuo, developed for semi-continuous rotifer cultivation, combines microalgae, probiotics and ammonia chelator for maintenance of water quality. In liquid concentrate typology, the premium product P. Rot Batch (18% dry weight - PS) was developed for rotifer culture in closed systems and batch production mode. Consisting of a modified hypersaline diluent solution, it allowed the optimization of the microbiological quality of the product and the culture, increasing the production efficiency. Recirculating aquaculture system's (P. Rot RAS) have recently started to be used for intensive rotifer production in semi-continuous production mode. Due to its complex filtration system, the product P. Rot RAS formula was developed, in liquid concentrate, with a lower density (15% PS) and an optimized salt solution (35 ppt). This formulation allowed the application in RotiRAS® systems (IDEE aquaculture, France) without filter clogging and promoted rotifer culture efficiency. Currently, there is a high demand for industrially produced microalgae feed solutions for bivalves as the industry is expanding due to the sudden need to increase the production of bivalve seed (post-larvae) in hatcheries, as climate change has promoted a sharp decline in natural recruitment. Existing commercial diets do not respect the species and proportions appropriate for the nutrition of bivalves. Therefore, the BIV 1 and BIV 2 diets were developed in low density liquid concentrate (8 % PS), for the rearing of post-larvae and juveniles and juveniles, respectively. Subsequently, the BIV 1 diet was optimized in terms of diluent salt solution. Through this internship, it was possible to develop 7 pilot products for aquaculture through which, analytical pipeline for the development of new microalgae products for aquaculture was established.
Description
Keywords
Microalgas Aquacultura Dietas Bivalves Larvicultura marinha