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An Insight into Sargassum muticum Cytoprotective Mechanisms against Oxidative Stress on a Human Cell In Vitro Model

2017Journal article Open access
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dc.contributor.authorPinteus, Susete
dc.contributor.authorLemos, Marco
dc.contributor.authorSilva, Joana
dc.contributor.authorAlves, Celso
dc.contributor.authorNeugebauer, Agnieszka
dc.contributor.authorFreitas, Rafaela
dc.contributor.authorDuarte, Adriana
dc.contributor.authorPedrosa, Rui
dc.date.accessioned2018-11-12T11:35:35Z
dc.date.available2018-11-12T11:35:35Z
dc.date.issued2017
dc.descriptionThis work was supported through funding provided by the Fundação para a Ciência e Tecnologia (FCT) Strategic Project UID/MAR/04292/2013 granted to MARE and by the Fundação para a Ciência e Tecnologia (FCT) R&D Project “Red2Discovery—The Red macro-algae Sphaerococcus coronopifolius and Asparagopsis armata as targets for marine drug Discovery” (P2020-PTDC/MAR-BIO/6149/2014). This work was also supported by the European Union through EASME Blue Labs project AMALIA—Algae-to-MArket Lab IdeAs (EASME/EMFF/2016/1.2.1.4/03/SI2.750419) and the Integrated Programme of SR&TD “Smart Valorization of Endogenous Marine Biological Resources Under a Changing Climate” (reference Centro-01-0145-FEDER-000018), co-funded by Centro 2020 program, Portugal 2020, European Union, through the European Regional Development Fund. Susete Pinteus, Joana Silva and Celso Alves are financial supported by a Grant from FCT—Fundação para a Ciência e Tecnologia (SFRH/BD/96203/2013, SFRH/BD/103255/2014 and SFRH/BD/97764/2013, respectively).
dc.description.abstractSargassum muticum is a brown seaweed with strong potential to be used as a functional food ingredient, mainly due to its antioxidant properties. It is widely used in traditional oriental medicine for the treatment of numerous diseases. Nevertheless, few studies have been conducted to add scientific evidence on its effects as well as on the mechanisms of action involved. In this work, the human cell line MCF-7 was used as an in vitro cellular model to evaluate the capability of Sargassum muticum enriched fractions to protect cells on an oxidative stress condition. The concentration of the bioactive compounds was obtained by vacuum liquid chromatography applied on methanol (M) and 1:1 methanol:dichloromethane (MD) crude extracts, resulting in seven enriched fractions from the M extraction (MF2-MF8), and eight fractions from the MD extraction (MDF1-MDF8). All fractions were tested for cytotoxic properties on MCF-7 cells and the nontoxic ones were tested for their capacity to blunt the damaging effects of hydrogen peroxide-induced oxidative stress. The nontoxic effects were also confirmed in 3T3 fibroblast cells as a nontumor cell line. The antioxidant potential of each fraction, as well as changes in the cell's real-time hydrogen peroxide production, in the mitochondrial membrane potential, and in Caspase-9 activity were evaluated. The results suggest that the protective effects evidenced by S. muticum can be related with the inhibition of hydrogen peroxide production and the inhibition of Caspase-9 activity.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.3390/md15110353pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.8/3639
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationNeuroprotective effects of molecules isolated from Bifurcaria bifurcata and Codium tomentosum in an in vitro and in vivo Parkinson Disease model.
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAntioxidantspt_PT
dc.subjectBiological Productspt_PT
dc.subjectCaspase 9pt_PT
dc.subjectHydrogen Peroxidept_PT
dc.subjectMCF-7 Cellspt_PT
dc.subjectMembrane Potential, Mitochondrialpt_PT
dc.subjectOxidative Stresspt_PT
dc.subjectSargassumpt_PT
dc.titleAn Insight into Sargassum muticum Cytoprotective Mechanisms against Oxidative Stress on a Human Cell In Vitro Modelpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleNeuroprotective effects of molecules isolated from Bifurcaria bifurcata and Codium tomentosum in an in vitro and in vivo Parkinson Disease model.
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMAR%2F04292%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F96203%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/POR_CENTRO/SFRH%2FBD%2F103255%2F2014/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F97764%2F2013/PT
oaire.citation.issue11pt_PT
oaire.citation.startPage353pt_PT
oaire.citation.volume15pt_PT
oaire.fundingStream5876
oaire.fundingStreamSFRH
oaire.fundingStreamPOR_CENTRO
oaire.fundingStreamSFRH
person.familyNamePinteus
person.familyNameLemos
person.familyNameSilva
person.familyNameAlves
person.familyNameFreitas
person.familyNamePedrosa
person.givenNameSusete
person.givenNameMarco
person.givenNameJoana
person.givenNameCelso
person.givenNameRafaela
person.givenNameRui
person.identifier159292
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person.identifier.ciencia-id591B-9204-DEDB
person.identifier.ciencia-id3817-33DE-919E
person.identifier.orcid0000-0002-3290-3111
person.identifier.orcid0000-0001-9887-1864
person.identifier.orcid0000-0003-1224-1699
person.identifier.orcid0000-0003-1581-2127
person.identifier.orcid0000-0002-0194-0470
person.identifier.orcid0000-0003-0970-0575
person.identifier.ridF-7951-2011
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person.identifier.ridB-4815-2015
person.identifier.scopus-author-id55655328300
person.identifier.scopus-author-id7006042884
person.identifier.scopus-author-id56370819800
person.identifier.scopus-author-id55654969100
person.identifier.scopus-author-id7005010300
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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