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Sex-steroids and hypolipidemic chemicals impacts on brown trout lipid and peroxisome signaling — Molecular, biochemical and morphological insights

2018Journal article Restricted access
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dc.contributor.authorMadureira, Tânia Vieira
dc.contributor.authorMalhão, Fernanda
dc.contributor.authorSimões, Tiago
dc.contributor.authorPinheiro, Ivone
dc.contributor.authorLopes, Célia
dc.contributor.authorGonçalves, José F.
dc.contributor.authorUrbatzka, Ralph
dc.contributor.authorCastro, L. Filipe C.
dc.contributor.authorLemos, Marco F.L.
dc.contributor.authorRocha, Eduardo
dc.date.accessioned2019-10-25T11:10:00Z
dc.date.available2019-10-25T11:10:00Z
dc.date.issued2018
dc.description.abstractLipid metabolism involves complex pathways, which are regulated in a similar way across vertebrates. Hormonal and hypolipidemic deregulations cause lipid imbalance from fish to humans, but the underlying mechanisms are far from understood. This study explores the potential of using juvenile brown trout to evaluate the in vivo interferences caused by estrogenic (17α-ethinylestradiol - EE2), androgenic (testosterone - T), and hypolipidemic (clofibrate - CLF) compounds in lipidic and/or peroxisomal pathways. Studied endpoints were from blood/plasma biochemistry, plasma fatty acid profile, ultrastructure of hepatocytes and abundance of their peroxisomes to mRNA expression in the liver. Both T and CLF caused minimal effects when compared to EE2. Estrogenized fish had significantly higher hepatosomatic indexes, increased triglycerides and very-low density lipoproteins (VLDL) in plasma, compared with solvent control. Morphologically, EE2 fish showed increased lipid droplets in hepatocytes, and EE2 and T reduced volume density of peroxisomes in relation to the hepatic parenchyma. Polyunsaturated fatty acids (PUFA) in plasma, namely n-3 PUFA, increased with EE2. EE2 animals had increased mRNA levels of vitellogenin A (VtgA), estrogen receptor alpha (ERα), peroxisome proliferator-activated receptor alpha (PPARα), PPARαBa and acyl-CoA long chain synthetase 1 (Acsl1), while ERβ-1, acyl-CoA oxidase 1-3I (Acox1-3I), Acox3, PPARγ, catalase (Cat), urate oxidase (Uox), fatty acid binding protein 1 (Fabp1) and apolipoprotein AI (ApoAI) were down-regulated. In summary, in vivo EE2 exposure altered lipid metabolism and peroxisome dynamics in brown trout, namely by changing the mRNA levels of several genes. Our model can be used to study possible organism-level impacts, viz. in gonadogenesis.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationSex-steroids and hypolipidemic chemicals impacts on brown trout lipid and peroxisome signaling - Molecular, biochemical and morphological insights. Madureira TV, Malhão F, Simões T, Pinheiro I, Lopes C, Gonçalves JF, Urbatzka R, Castro LFC, Lemos MFL, Rocha E. Comp Biochem Physiol C Toxicol Pharmacol. 2018 Oct;212:1-17. doi: 10.1016/j.cbpc.2018.06.001. Epub 2018 Jun 7. PMID: 29885532pt_PT
dc.identifier.doi10.1016/j.cbpc.2018.06.001
dc.identifier.urihttp://hdl.handle.net/10400.8/4244
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.subjectFatty acidspt_PT
dc.subjectBrown troutpt_PT
dc.subjectPeroxisomespt_PT
dc.subjectClofibratept_PT
dc.subjectTestosteronept_PT
dc.subjectEE2pt_PT
dc.titleSex-steroids and hypolipidemic chemicals impacts on brown trout lipid and peroxisome signaling — Molecular, biochemical and morphological insightspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage17pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titleComparative Biochemistry and Physiology - Part C: Toxicology and Pharmacologypt_PT
oaire.citation.volume212pt_PT
person.familyNameSimões
person.familyNameLemos
person.givenNameTiago
person.givenNameMarco
person.identifier996337
person.identifier.ciencia-id0D1D-8D1A-A883
person.identifier.ciencia-id971F-ACCA-C0D1
person.identifier.orcid0000-0002-6107-9790
person.identifier.orcid0000-0001-9887-1864
person.identifier.ridF-7951-2011
person.identifier.scopus-author-id16302507900
person.identifier.scopus-author-id7006042884
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication5699372e-8c6b-40ff-b5e9-af28324980e3
relation.isAuthorOfPublicationf21e5540-df76-43e9-ad64-93edd70da1f1
relation.isAuthorOfPublication.latestForDiscovery5699372e-8c6b-40ff-b5e9-af28324980e3

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