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Development of a new multiplex-PCR tool for the simultaneous detection of the fish pathogens Vibrio alginolyticus, Vibrio anguillarum, Vibrio harveyi and Edwardsiella tarda

2017-01-31Artigo científico Acesso aberto
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datacite.subject.sdg12:Produção e Consumo Sustentáveis
datacite.subject.sdg13:Ação Climática
datacite.subject.sdg14:Proteger a Vida Marinha
dc.contributor.authorPinto, Micaela Ferreira
dc.contributor.authorBaptista, Teresa
dc.contributor.authorAfonso, Clélia Correia Neves
dc.date.accessioned2026-03-24T16:12:54Z
dc.date.available2026-03-24T16:12:54Z
dc.date.issued2017-01-31
dc.description.abstractDisease assessment and management in cultured aquatic animals is a major concern in commercial aquaculture. Disease outbreaks have direct effects on fish production, causing serious economic losses in this industry. This can be overcome by early detection through molecular high sensitivity tools such as PCR (polymerase chain reaction). One of the most critical steps in the study of bacterial fish diseases is the precise identification of the infectious agent. Considering the damage that some bacteria can cause to fish and humans, the development of a rapid detection method for the four target species that demonstrates to be simple, accurate and low cost is an essential step for the prevention and early treatment of these diseases. Edwardsiella tarda (ACC 36.1), Vibrio harveyi (DSM 19023), Vibrio anguillarum (AQV 55.1) and Vibrio alginolyticus (CECT 521) were selected as targets of a multiplex PCR tool. The multiplex PCR reactions were performed in various reaction conditions, including different annealing temperatures (between 49 °C and 55 °C) and changes in the MgCl2 concentration from 2mMto 8 mM. Best results were obtained at 51 °C and MgCl2 concentration from 4mM to 6 mM. Primers were tested using purified DNA from the respective bacterial strains, yielding bands at expected sizes, which can be identified on agarose gels with clearness and without overlapping sizes. Results indicate that this multiplex PCR tool is suitable for the detection of target pathogens and may, in the future, have extended practical application in commercial aquaculture.eng
dc.description.sponsorshipThis study had the support of Fundação para a Ciência e Tecnologia (FCT), through the strategic project UID/MAR/04292/2013 granted to MARE.
dc.identifier.citationDevelopment of a new multiplex-PCR tool for the simultaneous detection of the fish pathogens Vibrio alginolyticus, Vibrio anguillarum, Vibrio harveyi and Edwardsiella tarda Micaela Ferreira Pinto, Teresa Baptista, Clélia Correia Neves Afonso Aquat. Living Resour. 30 4 (2017) DOI: 10.1051/alr/2017005
dc.identifier.doi10.1051/alr/2017005
dc.identifier.issn1765-2952
dc.identifier.urihttp://hdl.handle.net/10400.8/15973
dc.language.isoeng
dc.peerreviewedyes
dc.publisherEDP Sciences
dc.relationMARE - Marine and Environmental Sciences Centre
dc.relation.hasversionhttps://www.alr-journal.org/articles/alr/full_html/2017/01/alr160119/alr160119.html
dc.relation.ispartofAquatic Living Resources
dc.rights.uriN/A
dc.subjectDisease diagnostic
dc.subjectMultiplex-PCR
dc.subjectVibrio alginolyticus
dc.subjectVibrio anguillarum
dc.subjectVibrio harveyi
dc.subjectEdwardsiella tarda
dc.titleDevelopment of a new multiplex-PCR tool for the simultaneous detection of the fish pathogens Vibrio alginolyticus, Vibrio anguillarum, Vibrio harveyi and Edwardsiella tardaeng
dc.typejournal article
dspace.entity.typePublication
oaire.awardNumberUID/MAR/04292/2013
oaire.awardTitleMARE - Marine and Environmental Sciences Centre
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FMAR%2F04292%2F2013/PT
oaire.citation.endPage6
oaire.citation.issue4
oaire.citation.startPage1
oaire.citation.titleAquatic Living Resources
oaire.citation.volume30
oaire.fundingStream6817 - DCRRNI ID
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameBaptista
person.familyNameAfonso
person.givenNameTeresa
person.givenNameClélia
person.identifier153274
person.identifier.ciencia-idA61F-25C1-034A
person.identifier.ciencia-idC512-13C3-50DD
person.identifier.orcid0000-0003-1168-7461
person.identifier.orcid0000-0002-4167-5237
person.identifier.ridB-1211-2016
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
relation.isAuthorOfPublication3f0c36c9-c1c4-44e5-801c-b307dbe5625a
relation.isAuthorOfPublication4876cc93-8744-4885-ae53-7b552036195d
relation.isAuthorOfPublication.latestForDiscovery3f0c36c9-c1c4-44e5-801c-b307dbe5625a
relation.isProjectOfPublicationeb4d28f0-4a5c-4473-a445-aef6f6cb124d
relation.isProjectOfPublication.latestForDiscoveryeb4d28f0-4a5c-4473-a445-aef6f6cb124d

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