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An integrative assessment to determine the genotoxic hazard of estuarine sediments: combining cell and whole-organism responses

2014-12-10Artigo científico Acesso aberto
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datacite.subject.fosCiências Médicas::Medicina Clínica
datacite.subject.fosCiências Naturais::Ciências Biológicas
datacite.subject.fosCiências Médicas::Biotecnologia Médica
datacite.subject.sdg12:Produção e Consumo Sustentáveis
datacite.subject.sdg14:Proteger a Vida Marinha
dc.contributor.authorCosta, Pedro M.
dc.contributor.authorPinto, Miguel
dc.contributor.authorVicente, Ana M.
dc.contributor.authorGonçalves, Cátia
dc.contributor.authorRodrigo, Ana P.
dc.contributor.authorLouro, Henriqueta
dc.contributor.authorCosta, Maria H.
dc.contributor.authorCaeiro, Sandra
dc.contributor.authorSilva, Maria J.
dc.date.accessioned2026-07-24T14:49:44Z
dc.date.available2026-07-24T14:49:44Z
dc.date.issued2014-12-10
dc.description.abstractThe application of the Comet assay in environmental monitoring remains challenging in face of the complexity of environmental stressors, e.g., when dealing with estuarine sediments, that hampers the drawing of cause-effect relationships. Although the in vitro Comet assay may circumvent confounding factors, its application in environmental risk assessment (ERA) still needs validation. As such, the present work aims at integrating genotoxicity and oxidative DNA damage induced by sediment-bound toxicants in HepG2 cells with oxidative stress-related effects observed in three species collected from an impacted estuary. Distinct patterns were observed in cells exposed to crude mixtures of sediment contaminants from the urban/industrial area comparatively to the ones from the rural/riverine area of the estuary, with respect to oxidative DNA damage and oxidative DNA damage. The extracts obtained with the most polar solvent and the crude extracts caused the most significant oxidative DNA damage in HepG2 cells, as measured by the formamidopyrimidine-DNA glycosylase (FPG)-modified Comet assay. This observation suggests that metals and unknown toxicants more hydrophilic than polycyclic aromatic hydrocarbons may be important causative agents, especially in samples from the rural part of the estuary, where oxidative DNA damage was the most significant. Clams, sole, and cuttlefish responded differentially to environmental agents triggering oxidative stress, albeit yielding results accordant with the oxidative DNA damage observed in HepG2 cells. Overall, the integration of in vivo biomarker responses and Comet assay data in HepG2 cells yielded a comparable pattern, indicating that the in vitro FPG-modified Comet assay may be an effective and complementary line-of-evidence in ERA even in particularly challenging, natural, scenarios such as estuarine environments.eng
dc.description.sponsorshipPedro M. Costa was supported by the Portuguese Science and Technology Foundation (FCT) through the grant SFRH/BPD/72564/2010. The present research was financed by FCT and co-financed by the European Community FEDER through the program COMPETE (project reference PTDC/SAU-ESA/100107/2008). The authors are also thankful to S. Carreira, M. Martins, and J. Lobo (IMAR).
dc.identifier.citationCosta, P. M.; Pinto, M.; Vicente, A. M.; Gonçalves, C.; Rodrigo, A.P.; Louro, H.; Costa, M.H.; Caeiro, S. and Silva, M. J. (2014) An integrative assessment to determine the genotoxic hazard of estuarine sediments: combining cell and whole-organism responses. Front. Genet. 5:437. doi: https://doi.org/10.3389/fgene.2014.00437.
dc.identifier.doi10.3389/fgene.2014.00437
dc.identifier.eissn1664-8021
dc.identifier.urihttp://hdl.handle.net/10400.8/16675
dc.language.isoeng
dc.peerreviewedyes
dc.publisherFrontiers Media
dc.relationANALYSIS OF THE GENOTOXIC POTENTIAL OF ESTUARINE SEDIMENTS AND THE DNA REPAIR AND APOPTOSIS-RELATED RESPONSES IN HUMAN HEPG2 CELLS
dc.relationHERA - Environmental Risk Assessment of a contaminated estuarine environment: A case study
dc.relation.hasversionhttps://www.frontiersin.org/journals/genetics/articles/10.3389/fgene.2014.00437/full
dc.relation.ispartofFrontiers in Genetics
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectCometassay
dc.subjectenvironmentalriskassessment
dc.subjectsedimentcontamination
dc.subjectoxidativestress
dc.subjectHepG2cells
dc.titleAn integrative assessment to determine the genotoxic hazard of estuarine sediments: combining cell and whole-organism responseseng
dc.typejournal article
dspace.entity.typePublication
oaire.awardNumberSFRH/BPD/72564/2010
oaire.awardNumberPTDC/SAU-ESA/100107/2008
oaire.awardTitleANALYSIS OF THE GENOTOXIC POTENTIAL OF ESTUARINE SEDIMENTS AND THE DNA REPAIR AND APOPTOSIS-RELATED RESPONSES IN HUMAN HEPG2 CELLS
oaire.awardTitleHERA - Environmental Risk Assessment of a contaminated estuarine environment: A case study
oaire.awardURIhttp://hdl.handle.net/10400.8/16536
oaire.awardURIhttp://hdl.handle.net/10400.8/16673
oaire.citation.endPage12
oaire.citation.startPage1
oaire.citation.titleFrontiers in Genetics
oaire.citation.volume5
oaire.fundingStreamConcurso para Projectos de I&D em todos os Domínios Científicos - 2008
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameCosta
person.givenNamePedro M.
person.identifier.orcid0000-0002-9072-3522
relation.isAuthorOfPublication01d7c7c5-8ff1-48c8-a0ca-576b1e89a02c
relation.isAuthorOfPublication.latestForDiscovery01d7c7c5-8ff1-48c8-a0ca-576b1e89a02c
relation.isProjectOfPublication94e4b0a6-ce4a-42af-8b23-8fa74b2aabfc
relation.isProjectOfPublication9cb5a257-efba-4f8a-a19a-a413a615a273
relation.isProjectOfPublication.latestForDiscovery94e4b0a6-ce4a-42af-8b23-8fa74b2aabfc

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The application of the Comet assay in environmental monitoring remains challenging in face of the complexity of environmental stressors, e.g., when dealing with estuarine sediments, that hampers the drawing of cause-effect relationships. Although the in vitro Comet assay may circumvent confounding factors, its application in environmental risk assessment (ERA) still needs validation. As such, the present work aims at integrating genotoxicity and oxidative DNA damage induced by sediment-bound toxicants in HepG2 cells with oxidative stress-related effects observed in three species collected from an impacted estuary. Distinct patterns were observed in cells exposed to crude mixtures of sediment contaminants from the urban/industrial area comparatively to the ones from the rural/riverine area of the estuary, with respect to oxidative DNA damage and oxidative DNA damage. The extracts obtained with the most polar solvent and the crude extracts caused the most significant oxidative DNA damage in HepG2 cells, as measured by the formamidopyrimidine-DNA glycosylase (FPG)-modified Comet assay. This observation suggests that metals and unknown toxicants more hydrophilic than polycyclic aromatic hydrocarbons may be important causative agents, especially in samples from the rural part of the estuary, where oxidative DNA damage was the most significant. Clams, sole, and cuttlefish responded differentially to environmental agents triggering oxidative stress, albeit yielding results accordant with the oxidative DNA damage observed in HepG2 cells. Overall, the integration of in vivo biomarker responses and Comet assay data in HepG2 cells yielded a comparable pattern, indicating that the in vitro FPG-modified Comet assay may be an effective and complementary line-of-evidence in ERA even in particularly challenging, natural, scenarios such as estuarine environments.
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