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Effects of thermal stress on the immune and oxidative stress responses of juvenile sea cucumber Holothuria scabra

2017Journal article Restricted access
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datacite.subject.sdg12:Produção e Consumo Sustentáveis
datacite.subject.sdg13:Ação Climática
datacite.subject.sdg14:Proteger a Vida Marinha
dc.contributor.authorKamyab, Elham
dc.contributor.authorKühnhold, Holger
dc.contributor.authorNovais, Sara C.
dc.contributor.authorAlves, Luís M. F.
dc.contributor.authorIndriana, Lisa
dc.contributor.authorKunzmann, Andreas
dc.contributor.authorSlater, Matthew
dc.contributor.authorLemos, Marco F. L.
dc.date.accessioned2026-01-21T11:48:24Z
dc.date.available2026-01-21T11:48:24Z
dc.date.issued2017
dc.description.abstractHolothuria scabra is the most valued and cultured tropical sea cucumber, given the great demand of this species for human consumption. However, despite its ecological and economic relevance, little is known regarding its immune responses under thermal stress. Here, the main goal was to study the response of sea cucumbers to temperature stress, assessing sub-organismal alterations and acclimation capacities of juveniles to temperature changes. After changing temperature (1 °C/day) for 6 days, organisms were exposed to temperature conditions of 21 °C (cold), 27 °C (control), and 33 °C (warm) over a 30 day period. At each 15-day interval (T0, T15, and T30), six replicates per condition were killed for biochemical analysis. Immune responses were addressed by studying the activity of phenoloxidase (PO) and prophenoloxidase (ProPO) in the coelomic fluid. Antioxidant defence responses-catalase (CAT), superoxide dismutase (SOD), and glutathione reductase (GR) enzymatic activities-were measured in the muscle and respiratory tree tissues, whereas oxidative damage was evaluated by measuring levels of superoxide radicals (ROS), DNA-strand breaks and lipid peroxidation (LPO). Juvenile H. scabra increased SOD and PO activities when temperature was elevated, and revealed low levels of ROS and damage in both cold and warm treatments throughout the experiment, confirming the organism's moderate thermal stress. After the short acclimation period, the immune and antioxidant responses prevented damage and maintained homeostasis. This multi-biomarker approach highlights its usefulness to monitor the health of H. scabra and to gain insight concerning the use of this high-valued species in global-scale aquaculture from different temperature regions.eng
dc.description.sponsorshipThis study had the support of the Fundação para a Ciência e a Tecnologia (FCT) Strategic Project UID/MAR/04292/2013 granted to MARE, and from an FCT and Deutscher Akademischer Austauschdienst (DAAD) program for bilateral coop eration funding. Sara Novais was supported by Fundação para a Ciên cia e Tecnologia through the research Grant SFRH/BPD/94500/2013
dc.identifier.citationKamyab E, Kühnhold H, Novais SC, Alves LM, Indriana L, Kunzmann A, Slater M, Lemos MF. Effects of thermal stress on the immune and oxidative stress responses of juvenile sea cucumber Holothuria scabra. J Comp Physiol B. 2017 Jan;187(1):51-61. doi: 10.1007/s00360-016-1015-z. Epub 2016 Jul 21. PMID: 27439718
dc.identifier.doi10.1007/s00360-016-1015-z
dc.identifier.issn0174-1578
dc.identifier.issn1432-136X
dc.identifier.urihttp://hdl.handle.net/10400.8/15435
dc.language.isoeng
dc.peerreviewedyes
dc.publisherSpringer Science and Business Media LLC
dc.relationMARE - Marine and Environmental Sciences Centre
dc.relationENVIRONOME - INTEGRATING OMICS IN ECOTOXICOLOGY: TOOLS FOR ENVIRONMENTAL RISK ASSESSMENT
dc.relation.hasversionhttps://www.springer.com/journal/360
dc.relation.ispartofJournal of Comparative Physiology B
dc.rights.uriN/A
dc.subjectAcclimation
dc.subjectAntioxidant responses
dc.subjectBiomarkers
dc.subjectClimate change
dc.subjectEnvironmental stress
dc.subjectTropical aquaculture
dc.titleEffects of thermal stress on the immune and oxidative stress responses of juvenile sea cucumber Holothuria scabraeng
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleMARE - Marine and Environmental Sciences Centre
oaire.awardTitleENVIRONOME - INTEGRATING OMICS IN ECOTOXICOLOGY: TOOLS FOR ENVIRONMENTAL RISK ASSESSMENT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FMAR%2F04292%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/FARH/SFRH%2FBPD%2F94500%2F2013/PT
oaire.citation.endPage61
oaire.citation.issue1
oaire.citation.startPage51
oaire.citation.titleJournal of Comparative Physiology B: Biochemical, Systems, and Environmental Physiology
oaire.citation.volume187
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamFARH
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameNovais
person.familyNameLemos
person.givenNameSara
person.givenNameMarco
person.identifier996337
person.identifier.ciencia-id2216-A2E0-A118
person.identifier.ciencia-id971F-ACCA-C0D1
person.identifier.orcid0000-0003-1306-3396
person.identifier.orcid0000-0001-9887-1864
person.identifier.ridA-9032-2012
person.identifier.ridF-7951-2011
person.identifier.scopus-author-id23025463300
person.identifier.scopus-author-id7006042884
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
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relation.isAuthorOfPublicationf21e5540-df76-43e9-ad64-93edd70da1f1
relation.isAuthorOfPublication.latestForDiscoveryde69a91d-24ba-4155-ad23-76367cca2232
relation.isProjectOfPublicationeb4d28f0-4a5c-4473-a445-aef6f6cb124d
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