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Bidirectional flow of action potentials in axons drives activity dynamics in neuronal cultures

2021-12-29Journal article Open access
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dc.contributor.authorMateus, J. C.
dc.contributor.authorLopes, C. D. F.
dc.contributor.authorAroso, M.
dc.contributor.authorCosta, A. R.
dc.contributor.authorGerós, A.
dc.contributor.authorMeneses, J.
dc.contributor.authorFaria, P.
dc.contributor.authorNeto, E.
dc.contributor.authorLamghari, M.
dc.contributor.authorSousa, M. M.
dc.contributor.authorAguiar, P
dc.date.accessioned2023-04-13T15:40:44Z
dc.date.available2023-04-13T15:40:44Z
dc.date.issued2021-12-29
dc.description.abstractObjective. Recent technological advances are revealing the complex physiology of the axon and challenging long-standing assumptions. Namely, while most action potential (AP) initiation occurs at the axon initial segment in central nervous system neurons, initiation in distal parts of the axon has been reported to occur in both physiological and pathological conditions. The functional role of these ectopic APs, if exists, is still not clear, nor its impact on network activity dynamics. Approach. Using an electrophysiology platform specifically designed for assessing axonal conduction we show here for the first time regular and effective bidirectional axonal conduction in hippocampal and dorsal root ganglia cultures. We investigate and characterize this bidirectional propagation both in physiological conditions and after distal axotomy. Main results. A significant fraction of APs are not coming from the canonical synapse-dendrite-soma signal flow, but instead from signals originating at the distal axon. Importantly, antidromic APs may carry information and can have a functional impact on the neuron, as they consistently depolarize the soma. Thus, plasticity or gene transduction mechanisms triggered by soma depolarization can also be affected by these antidromic APs. Conduction velocity is asymmetrical, with antidromic conduction being slower than orthodromic. Significance. Altogether these findings have important implications for the study of neuronal function in vitro, reshaping our understanding on how information flows in neuronal cultures.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationMateus JC, Lopes C, Aroso M, Costa AR, Gerós A, Meneses J, Faria P, Neto E, Lamghari M, Sousa MM, Aguiar P. Bidirectional flow of action potentials in axons drives activity dynamics in neuronal cultures. J Neural Eng. 2021 Dec 29;18(6). doi: 10.1088/1741-2552/ac41db. PMID: 34891149pt_PT
dc.identifier.doi10.1088/1741-2552/ac41dbpt_PT
dc.identifier.urihttp://hdl.handle.net/10400.8/8382
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherIOPpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectMicrofluidicspt_PT
dc.subjectMicroelectrode arraypt_PT
dc.subjectDorsal root gangliapt_PT
dc.subjectHippocampal neuronspt_PT
dc.subjectEctopic action potentialpt_PT
dc.subjectBidirectional axonal conductionpt_PT
dc.subjectAxonal electrophysiologypt_PT
dc.titleBidirectional flow of action potentials in axons drives activity dynamics in neuronal culturespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.titleJournal of Neural Engineeringpt_PT
oaire.citation.volume18pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT

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