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Occurrence of tet(O/M/O) mosaic gene in tetracycline-resistant Campylobacter

2020Journal article Open access
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dc.contributor.authorHormeño, Lorena
dc.contributor.authorCampos, Maria J.
dc.contributor.authorVadillo, Santiago
dc.contributor.authorQuesada, Alberto
dc.date.accessioned2021-08-16T12:55:01Z
dc.date.available2021-08-16T12:55:01Z
dc.date.issued2020
dc.descriptionFunding: M.J.C. wishes to thank Fundação para a Ciência e Tecnologia (FCT), through the strategic project UID/MAR/04292/2013 granted to MARE and the Integrated Programme of SR&TD “Smart Valorization of Endogenous Marine Biological Resources Under a Changing Climate” (reference Centro-01-0145-FEDER- 000018), co-funded by the Centro 2020 program, Portugal 2020, European Union, through the European Regional Development Fund. Works in the A.Q. laboratory have been funded by projects AGL2012-39028-C03-03 and AGL2016-74882-C3-2-R (“Ministerio de Ciencia e Innovación, Gobierno de España”) and IB16073 (“Consejería de Economía e Infraestructuras, Junta de Extremadura” and FEDER).
dc.description.abstractCampylobacter is one of the most important microorganisms responsible for foodborne diseases in the EU. In this study, we investigated resistance to tetracycline in 139 Campylobacter jejuni and Campylobacter coli samples isolated from human clinical cases. From these, 110 were resistant to tetracycline, with MIC (minimal inhibitory concentration) varying in a range of 1 to >512 ug/mL, and 109 (78.4%) carried tet(O), a gene that confers resistance to tetracycline through the expression of a protein that confers protection to the ribosome. Amongst the tetracycline-resistant isolates, one C. jejuni (HCC30) was the only tet(O)-negative sample, presenting an MIC of 256 ug/mL. Instead, the mosaic gene tet(O/M/O) was found in HCC30 and, as far as we know, this is the first description of this chimeric gene originating from homologous recombination between tet(O) and tet(M). The previously described mosaic gene tet(O/32/O), also found in Campylobacter, presents a chimeric structure very similar to that of tet(O/M/O), affecting domains II and III of encoded proteins distantly related to the elongation factor G (EF-G). The tet(O/M/O) mosaic gene has been found in nucleotide databases in several genomes of Campylobacter isolated from different origins, indicating its frequent acquisition, even though it can be undetected through screening by PCR with specific tet(O) primers. In this work, we address the improvement of classical PCR to effciently diagnose the most prevalent tetracycline resistance determinants in Campylobacter, including tet(O/M/O), which should be taken into account in the optimization of campylobacteriosis treatments.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationHormeño, L.; Campos, M.J.; Vadillo, S.; Quesada, A. Occurrence of tet(O/M/O) Mosaic Gene in Tetracycline-Resistant Campylobacter. Microorganisms 2020, 8, 1710. https://doi.org/10.3390/microorganisms8111710pt_PT
dc.identifier.doi10.3390/microorganisms8111710pt_PT
dc.identifier.issn2076-2607
dc.identifier.urihttp://hdl.handle.net/10400.8/6113
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationMARE - Marine and Environmental Sciences Centre
dc.relation.publisherversionhttps://www.mdpi.com/2076-2607/8/11/1710pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectCampylobacter colipt_PT
dc.subjectCampylobacter jejunipt_PT
dc.subjectTetracycline resistancept_PT
dc.subjectTet genespt_PT
dc.subjectMosaic tet (O/MO)pt_PT
dc.titleOccurrence of tet(O/M/O) mosaic gene in tetracycline-resistant Campylobacterpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleMARE - Marine and Environmental Sciences Centre
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FMAR%2F04292%2F2013/PT
oaire.citation.issue11pt_PT
oaire.citation.titleMicroorganismspt_PT
oaire.citation.volume8pt_PT
oaire.fundingStream6817 - DCRRNI ID
person.familyNameCampos
person.givenNameMaria Jorge
person.identifier174191
person.identifier.ciencia-id2416-7867-2D40
person.identifier.orcid0000-0002-0883-0610
person.identifier.ridE-5591-2014
person.identifier.scopus-author-id55767069800
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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relation.isAuthorOfPublication.latestForDiscovery6d1bf3db-fdba-4bea-b65d-94491f841659
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