Optimised additive biomanufacturing system to produce hierarchical multi-tissue scaffolds for the treatment of joint diseases

Publicações

Toward Integrative Biomechanical Models of Osteochondral Tissues: A Multilayered Perspective

Publication . Filipe Ramos Cardoso da Silva, Bruna; Marco Domingos; Amado, Sandra; Dias, Juliana; Pascoal-Faria, Paula; Ana C. Maurício; Alves, Nuno

Understanding the complex mechanical behavior of osteochondral tissues in silico is essential for improving experimental models and advancing research in joint health and degeneration. This review provides a comprehensive analysis of the constitutive models currently used to represent the different layers of the osteochondral region, from articular cartilage to subchondral bone, including intermediate regions such as the tidemark and the calcified cartilage layer. Each layer exhibits unique structural and mechanical properties, necessitating a layer-specific modeling approach. Through critical comparison of existing mathematical models, the viscoelastic model is suggested as a pragmatic starting point for modeling articular cartilage zones, the tidemark, and the calcified cartilage layer, as it captures essential time-dependent behaviors such as creep and stress relaxation while ensuring computational efficiency for initial coupling studies. On the other hand, a linear elastic model was identified as an optimal starting point for both the subchondral bone plate and the subchondral trabecular bone, reflecting their dense and stiff nature, and providing a coherent framework for early-stage multilayer integration. This layered modeling approach enables the development of physiologically coherent and computationally efficient representations of osteochondral region modeling. Furthermore, by establishing a layer-specific modeling approach, this review paves the way for modular in silico simulations through the coupling of computational models. Such an integrative framework supports scaffold design, in vitro experimentation, preclinical validation, and the mechanobiological exploration of osteochondral degeneration and repair. These efforts are essential for deepening our understanding of tissue responses under both physiological and pathological conditions. Ultimately, this work provides a robust theoretical foundation for future in silico and in vitro studies aimed at advancing osteochondral tissue regeneration strategies.

2025-06-13Artigo científico

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Current Strategies to Produce 3D Electrospun-Based Wound Dressings for Skin Regeneration

Publication . Ferreira, Carolina A. M.; Lemos, Marco F. L.; Maurício, Ana Colette; Dias, Juliana R.

Tissue engineering-based wound dressings are a promising treatment approach that mimics the native skin microenvironment to promote effective healing and tissue regeneration. In fact, those advanced wound dressings can be made by electrospinning which has revolutionized the field of wound healing by developing biostructures that closely mimic the skin’s extracellular matrix (ECM). To date, most electrospinning research has focused on composition and materials rather than exploring advanced deposition strategies. Conventional electrospinning using random, aligned fibers, co-electrospinning, core-shell approaches, or redesigned collectors has achieved significant results in wound healing, which are discussed in this article. However, these approaches lack the development of realistic 3D structures. To achieve that, advancing approaches through the combination of electrospinning with different techniques such as gas foaming, short nanofiber assembly, or 3D printing significantly enhance skin regeneration of deep wounds (full-thickness and chronic wounds) compared to 2D structures. This review highlights the latest developments, design principles, and recent breakthroughs in electrospinning structures for skin regeneration and provides a fresh perspective for upcoming research in the field of 3D electrospun-based structures.

2025-07-02Artigo de revisão

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Palavras-chave

Optimised additive biomanufacturing system,Hierarchical multi-tissue scaffold,Tissue engineering,Joint diseases, Engineering and technology

Entidade financiadora

Fundação para a Ciência e a Tecnologia, I.P.
Fundação para a Ciência e a Tecnologia, I.P.
Fundação para a Ciência e a Tecnologia, I.P.

Programa de financiamento

3599-PPCDT
Concurso de Projetos de I&D em Todos os Domínios Científicos - 2022 - ICDT
Concurso de Projetos de I&D em Todos os Domínios Científicos - 2022

Número da atribuição

2022.10564.PTDC