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- Diabetes Zero - Centro de exercício clínicoPublication . Neves, Alex Martins; Morouço, Pedro Gil Frade
- Refletindo sobre a Prática Pedagógica do 1.º CEB e de Matemática e Ciências Naturais no 2.º CEB. Contributos da implementação de uma sequência didática sobre eletricidade, com enfoque numa perspetiva de ensino IBSE, para o desenvolvimento de conhecimentos e de competências de cooperação por alunos do 4.º ano de escolaridadePublication . Pires, Ana Teresa Santos; Rebelo, Isabel Sofia Godinho da SilvaO presente relatório, referente ao Mestrado em Ensino do 1.º Ciclo do Ensino Básico e de Matemática e Ciências Naturais no 2.º Ciclo do Ensino Básico, apresenta-se dividido em duas partes: a dimensão reflexiva e a dimensão investigativa. Na dimensão reflexiva expõe-se uma reflexão crítica e fundamentada relativamente às aprendizagens que foram sendo desenvolvidas ao nível pessoal, social e profissional, durante os contextos educativos de Prática Pedagógica em 1.º e 2.º Ciclo do Ensino Básico, bem como as principais dificuldades e receios sentidos. Na dimensão investigativa é apresentado um estudo de caso, de natureza qualitativa, com enfoque na implementação de uma metodologia de ensino por investigação (IBSE), assente no modelo dos 5 E´s, com crianças do 1.º Ciclo do Ensino Básico, mais propriamente do 4.º ano, procurando compreender as suas potencialidades no que concerne à aquisição de conhecimentos sobre eletricidade e ao desenvolvimento de competências de cooperação nos alunos. Desta forma, definiu-se a seguinte questão de investigação: De que forma uma sequência didática sobre eletricidade, organizada segundo o modelo dos 5E´s, pode promover o desenvolvimento de conhecimentos e de competências de cooperação pelos alunos? Os dados recolhidos evidenciam uma evolução por parte dos alunos, tanto ao nível das suas ideias sobre eletricidade, como do desenvolvimento de competências de cooperação.
- Comparison of cytochrome c with conventional biocatalysts in the degradation of environmental toxicantsPublication . Lagoa, Ricardo; Lopes, João M.; Rodrigues, J. Rui; Marques-da-Silva, DorindaPeroxidases and laccases are amongst the most attractive enzymes for the degradation of concerning pollutants like polycyclic aromatic hydrocarbons (PAHs) and organic dyes. In spite of the availability of high activity enzymes, their applicability is hindered by specificity and stability limitations in real environmental conditions, so more suitable biocatalysts are demanded. Cytochrome c (Cc) is best known as a protein electron carrier at mitochondria, but it also displays (pseudo-)peroxidase activity. In this work, we aimed to evaluate the potential of Cc as biocatalyst of PAHs’ and azo dyes’ degradation and compare it with two more conventional enzymes - plant peroxidases and fungi laccases. The studies were carried out with Cc from horse heart, horseradish peroxidase (HRP) and laccase from Trametes versicolor. The enzymes were tested with two major PAHs, anthracene and benzo[a]pyrene (BaP), and with methyl orange (MO) as a model azo dye. The enzyme-catalyzed oxidation of PAHs was determined by HPLC, and MO decolorization was followed in spectrophotometric kinetic assays. Several degradation studies were performed to assess the catalytic capacity at different pH and the effect of the redox mediator ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate)). Peroxidase reactions of HRP and Cc were initiated with 100 μM H2O2 (in media containing 100 μM DTPA as metal chelator). In pH 5 acetate buffer, the specific ABTS-oxidizing activity of Cc was much lower than that of laccase and HRP. However, these two enzymes lost almost all activity at pH 7, while Cc exhibited catalytic activity even at pH 8 (phosphate buffer). Decolorization assays showed laccase alone to be a weak catalyst of MO degradation, although addition of ABTS to the reaction media greatly accelerated the transformation. On the contrary, both HRP and Cc directly catalyzed MO decolorization, including at neutral pH, and the redox mediator offered no advantage. As for the PAHs, HRP and laccase catalysis benefited from the presence of ABTS in the media, but again Cc oxidized anthracene and BaP directly. Indeed, 24h incubation of BaP (1 mg/L) with Cc (0.1 mg/mL) resulted in the transformation of 70±4% of the PAH and generation of major reaction products different of the BaP quinones produced by laccase- and HRP-ABTS systems. This work disclosed catalytic properties of Cc different from laccase and HRP that are relevant for the design of new enzymatic remediation processes.
- Death cafés as a strategy to foster compassionate communities: Contributions for death and grief literacyPublication . Laranjeira, Carlos; Dixe, Maria dos Anjos; Querido, Ana; Stritch, Jennifer MoranThe death-positive movement, the most recent manifestation of the death awareness movement, contends that modern society is suffering from a “death taboo” and that people should talk more openly about death (Koksvik and Richards, 2021). This movement is striving to shift the dialogue about (and place of) death and dying into community spaces (Breen, 2020).
- What names for covert awareness? A systematic reviewPublication . Schnakers, Caroline; Bauer, Chase; Formisano, Rita; Noé, Enrique; Llorens, Roberto; Lejeune, Nicolas; Farisco, Michele; Teixeira, Liliana; Morrissey, Ann-Marie; De Marco, Sabrina; Veeramuthu, Vigneswaran; Ilina, Kseniya; Edlow, Brian L.; Gosseries, Olivia; Zandalasini, Matteo; De Bellis, Francesco; Thibaut, Aurore; Estraneo, AnnaAfter a severe brain injury, some patients do not fully recover consciousness and remain in a prolonged Disorder of Consciousness (DoC) such as the vegetative state/unresponsive wakefulness syndrome (VS/UWS) or the minimally conscious state (MCS). Patients in a VS/UWS open their eyes and present preserved autonomic functions, but they are not conscious and show only reflexive behaviors while the MCS is being characterized by the presence of inconsistent but clearly discernible behavioral signs of consciousness (e.g., visual tracking, command following) (The Multi-Society Task Force on Persistent Vegetative State, 1994; Giacino et al., 2002). More recently, the MCS has been subdivided in two clinical entities, MCS+ and MCS– (characterized by the presence/absence of command-following, intelligible verbalization, and intentional communication) supported by metabolic differences in areas associated with both consciousness and language (e.g., lower metabolism in the precuneus and thalamus and in the left middle temporal cortex in MCS-) (Thibaut et al., 2020). Prolonged DoCs are a relatively rare condition (estimated prevalence of 5,000–42,000 and 112,000–280,000 for VS/UWS and MCS, respectively, in the US) implying severe disability and complete dependence, which can last from 28 days to decades (Giacino et al., 2018)
- Set to fail: Affective dynamics in a resistance training program designed to reach muscle concentric failurePublication . Bastos, Vasco; Andrade, Ana Jesus; Rodrigues, Filipe; Monteiro, Diogo; Cid, Luis; Teixeira, Diogo SantosGrounded in hedonic assumptions, evidence suggests that people tend to engage in activities they consider pleasurable and enjoyable, while trying to avoid pain and displeasure. This suggests that the dynamic between positive and negative affect can influence current behavior and the intentions to continue performing. Regarding resistance training (RT), research focusing on how to promote a better affective response is still scarce and much needed. Given existing limitations and theoretical suggestions, a RT program was developed and applied to recreational exercisers in a quasi-experimental design aiming to (1) explore the affective response dynamic through an assessment after the last set of each exercise; and (2) analyze possible differences of preference and tolerance profiles in affective variables (core affect and enjoyment). For that purpose, 43 participants (21 male and 22 female; Mage = 34.69 ± 6.71 years; Mexperience = 8.32 ± 4.54 years; MBMI = 24.26 ± 2.64 kg/m2) accepted to participate in this study. Descriptive statistics, correlational, and group comparisons analyses were performed to provide evidence for proposed objectives. The present study showed that measures of affective valence/arousal applied immediately after a set represents a feasible and ecologically valid approach to tap core affect. Results presented evidence that recreationally trained exercisers in a common RT program would need a minimum of one measurement to assess the affective response. However, additional assessments could refine the understanding of exercise pleasurable experience. Results also suggest that exercisers with distinct profiles of preference/tolerance depicted differentiated patterns for the affective response, possibly justifying a distinct approach when promoting affective regulation.
- Understanding the Associations across Fibromyalgia-Related Fatigue, Depression, Anxiety, Self-Esteem Satisfaction with Life and Physical Activity in Portuguese and Brazilian Patients: A Structural Equation Modeling AnalysisPublication . Alvarez, Marcos; Albuquerque, Maria Luiza L.; Neiva, Henrique P.; Cid, Luis; Teixeira, Diogo S.; Matos, Rui; Antunes, Raul; Lúcio, Liane; Sant’Ana, Leandro; Monteiro, DiogoBackground and Objectives: Fibromyalgia are heterogeneous and differ from patient to patient; however, the most reported are general myalgia and at specific points associated with fatigue and certain psychological adversities. Physical activity can mitigate the effects of the symptoms. However, the associations between fibromyalgia-related fatigue, self-esteem, anxiety, depression, satisfaction with life and physical activity are unclear. Therefore, the aim of the present study was to understand the associations between these symptoms and whether there are differences between these associations across two distinct cultures. Materials and Methods: A total of 473 women aged between 28 and 75 years (M = 49.27; SD ± 8.28) completed five questionnaires about fibromyalgia-related fatigue, physical activity, anxiety, depression, self-esteem, and satisfaction with life. Results: Fibromyalgia-related fatigue was positively associated with depression and anxiety, depression and anxiety were negatively associated with self-esteem, self-esteem was positively associated with satisfaction with life, satisfaction with life was positively associated with physical activity and there were no differences in terms of the perceptions and associations of these variables between Portuguese and Brazilian patients. Conclusions: Our results showed the significant role of associations between these variables and a similarity in the perception and relationship of the variables between the two cultures.
- Recommendations for the Evaluation and Prescription of Strength Training in Individuals with Intellectual Difficulty and Development: Narrative ReviewPublication . Jacinto, Miguel; Monteiro, Diogo; Oliveira, Rafael; Brito, João; Vitorino, AnabelaThere are several benefits through the realization of physical exercise programs (EP) in individuals with Intellectual and Developmental Difficulties (DID), however, the literature presents a shortage in the implementation and a small number of studies with very different methodologies and types of training. Short-term interventions, a reduced number of longitudinal studies, methodological gaps and inappropriate training methods, hinder more explicit and consensual conclusions regarding the prescription and its results. However, EP programs, in particular strength training (ST), which has been associated with a reduced risk of cardiovascular and metabolic diseases, can be an asset for the individual with DID, given the prevalence of several comorbidities, namely hypertension, cholesterol and type II diabetes. The present study aims to identify the fundamental and structuring aspects for the prescription of ST, namely the assessment methods, intensity, duration, frequency, appropriate exercises and their relationship with the results, through the methodology using the narrative review, starting from the characterization of various ST programs implemented in individuals with DID. The results are presented in the form of recommendations for the evaluation and prescription of ST programs, in the population with DID.
- Assessing caregiving experience in COPD: Content validity of the Zarit Burden Interview in Canadian and Portuguese caregiversPublication . Selzler, Anne-Marie; Brooks, Dina; Marques, Alda; Souto-Miranda, Sara; Goldstein, Roger; Cruz, JoanaInformal caregivers play an important role in chronic disease management but their experience is often neglected. The objective of this study was to explore the content validity of the Zarit Burden Interview (ZBI) in caregivers of individuals with COPD in Canada and Portugal.
- Oral health-related quality of life in portuguese pre-school children: a cross-sectional studyPublication . Graça, S. R.; Dias, S.; Mendes, S.Purpose To study Oral Health related Quality of Life (OHRQoL) of pre-school children and its associated factors. Methods Cross-sectional study with sample data collected through an on-line questionnaire to a non-probabilistic sample. The questionnaire included the Portuguese version of the Early Childhood Oral Health Impact Scale (ECOHIS) and questions about sociodemographic characteristics, oral health behaviours, and reported oral health of the child. Data analyses included descriptive statistics, Mann–Whitney and Kruskal–Wallis tests and Linear regression (α=0.05). Results The sample consisted of 1475 parents of pre-school children (3–5 years-old), residing in Portugal. The mean ECOHIS total score was 1.5 (sd=3.4). In the bivariate analysis the child’s age, starting toothbrushing after one year of age, frequent intake of sugary food and drinks, previous appointment with an oral health professional, all reported of oral health problems, and a negative perception of oral health were negatively associated with OHRQoL (p<0,05). In the regression model the factors that most contributed to a worse quality of life were dental caries and abscess, contributing to an ECOHIS average increase of 2.56 and 3.34, respectively. Conclusions The studied population presented a good OHRQoL, with the item related to pain being the most relevant for the ECOHIS score. Worst OHRQoL was found in older children, whose parents negatively rated the children's oral health and with reported dental caries, trauma, and abscess.