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- Numerical-experimental analysis of the permeability-porosity relationship in triply periodic minimal surfaces scaffoldsPublication . Pires, T.; Santos, J.; Ruben, R. B.; Gouveia, B.; Castro, A. P. G.; Fernandes, P. R.Bone Tissue Engineering has been focusing on improving the current methods for bone repair, being the use of scaffolds presented as an upgrade to traditional surgery techniques. Scaffolds are artificially porous matrices, meant to promote cell seeding and proliferation, being these properties influenced by the permeability of the structure. This work employed experimental pressure drop tests and Computational Fluid Dynamics models to assess permeability (and fluid streamlines) within different triply periodic minimal surfaces scaffold geometries (Schwarz D, Gyroid and Schwarz P). The pressure outputs from the computational analysis presented a good correlation with the experimental results, with R2 equal to 0.903; they have also shown that a lower porosity may not mean a lower permeability if the geometry is altered, such as the difference between 60% porous Gyroid scaffolds (8.1*10-9 mm2) and 70% porous Schwarz D scaffolds (7.1*10-9 mm2). Fluid streamlines revealed how the Gyroid geometries are the most appropriate design for most bone tissue engineering applications, due to their consistent fluid permeation, followed by Schwarz D. The Schwarz P geometries have shown flat streamlines and significant variation of the permeability with the porosity (an increase of 10% in their porosity lead to an increase in the permeability from 5.1*10-9 mm2 to 11.7*10-9 mm2), which would imply a poor environment for cell seeding and proliferation.
- Effect of Plasmid DNA Size on Chitosan or Polyethyleneimine Polyplexes FormulationPublication . Valente, J. F. A.; Pereira, P.; Sousa, A.; Queiroz, J. A.; Sousa, F.Gene therapy could be simply defined as a strategy for the introduction of a functional copy of desired genes in patients, to correct some specific mutation and potentially treat the respective disorder. However, this straightforward definition hides very complex processes related to the design and preparation of the therapeutic genes, as well as the development of suitable gene delivery systems. Within non-viral vectors, polymeric nanocarriers have offered an ideal platform to be applied as gene delivery systems. Concerning this, the main goal of the study was to do a systematic evaluation on the formulation of pDNA delivery systems based on the complexation of different sized plasmids with chitosan (CH) or polyethyleneimine (PEI) polymers to search for the best option regarding encapsulation efficiency, surface charge, size, and delivery ability. The cytotoxicity and the transfection efficiency of these systems were accessed and, for the best p53 encoding pDNA nanosystems, the ability to promote protein expression was also evaluated. Overall, it was showed that CH polyplexes are more efficient on transfection when compared with the PEI polyplexes, resulting in higher P53 protein expression. Cells transfected with CH/p53-pDNA polyplexes presented an increase of around 54.2% on P53 expression, while the transfection with the PEI/p53-pDNA polyplexes resulted in a 32% increase.
- A Novel Specimen Produced by Additive Manufacturing for Pure Plane Strain Fatigue Crack Growth StudiesPublication . Jesus, Joel de; Borges, Micael; Antunes, Fernando; Ferreira, José; Reis, Luis; Capela, CarlosFatigue crack growth is usually studied using C(T) or M(T) specimens with through-thickness cracks. The objective of the present study is to propose a cylindrical specimen with central crack, produced by additive manufacturing. This geometry allows to have pure plane strain state along the whole crack front, avoiding the complexities associated with corner points, crack shape, and variation of crack closure along crack front. Additionally, this geometry may be used to develop studies in vacuum, avoiding expensive vacuum equipment, since the air is not in contact with the crack front. Cylindrical specimens of Ti6Al4V titanium alloy were produced by Selective Laser Melting and tested at a stress ratio R = 0. Marking with overloads was the solution adopted to measure the length of the internal cracks. The fracture surfaces presented circular crack fronts and the da/dN-DK curves showed a great influence of atmosphere on fatigue crack growth. An average difference of 50% was found between the results in air and vacuum. Therefore, this geometry with internal crack is an interesting alternative to through-thickness geometries.