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Browsing by Author "Moura, Carla Sofia"

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  • Combination of 3D Extruded-based Poly (ɛ-caprolactone) Scaffolds with Mesenchymal Stem/Stromal Cells: Strategy Optimization
    Publication . Moura, Carla Sofia; Silva, Cláudia Lobato da; Bartolo, Paulo Jorge; Ferreira, Frederico Castelo
    This study uses a combination of 3D extruded-based biodegradable scaffolds and human bone marrow-derived mesenchymal stem/stromal cells to understand how scaffold properties can contribute for efficient cell seeding, proliferation and migration. Highly interconnected porous poly(ɛ-caprolactone) scaffolds were produced by a layer-by-layer extrusion. Two configurations were studied with larger (390 μm) or smaller pore size (190 μm) at the centre. MSC, seeded on the top centre of the scaffold surface, increased 5 folds after 13 days of cultivation for both configurations, allowing us to conclude that in this range of pore sizes pose no limitations for cell migration are observed.
    2015Journal article Open access Show more
  • Effects of Different Fibre Alignments and Bioactive Coatings on Mesenchymal Stem/Stromal Cell Adhesion and Proliferation in Poly (ɛ-caprolactone) Scaffolds towards Cartilage Repair
    Publication . Silva, João Carlos; Moura, Carla Sofia; Alves, Nuno; Cabral, Joaquim Manuel Sampaio; Ferreira, Frederico Castelo
    In this work, 3D biodegradable poly (ɛ-caprolactone) scaffolds with high porosity and interconnectivity were produced by extrusion and characterized in terms of their structural and mechanical properties. The effects of scaffold fibre alignment (0-45° and 0-90°) and of different adhesive coatings (Fibronectin, Gelatin and commercially available CELLstart™) on bone marrow mesenchymal stem/stromal cells adhesion, migration and proliferation were evaluated using AlamarBlue™ assay. Fibronectin and CELLstart™ coated poly (ɛ-caprolactone) scaffolds presented an improvement in cell adhesion of approximately 2-fold relatively to the non-treated scaffolds, independently of fibre alignment. Overall, all the conditions studied promoted the growth and migration of bone marrow mesenchymal stem cells in the fabricated poly(ɛ-caprolactone) scaffolds, without affecting their chondrogenic differentiation and extracellular matrix deposition evaluated by Alcian Blue staining.
    2017Journal article Open access Show more