Repositório IC-Online
Repositório Científico da Instituição
Entradas recentes
Selective Inhibition of Clade A Phosphatases Type 2C by PYR/PYL/RCAR Abscisic Acid Receptors
Publication . Antoni, Regina; Gonzalez-Guzman, Miguel; Rodriguez, Lesia; Rodrigues, Américo; Pizzio, Gaston A.; Rodriguez, Pedro L.
Abstract
Clade A protein phosphatases type 2C (PP2Cs) are negative regulators of abscisic acid (ABA) signaling that are inhibited in an ABA-dependent manner by PYRABACTIN RESISTANCE1 (PYR1)/PYR1-LIKE (PYL)/REGULATORY COMPONENTS OF ABA RECEPTORS (RCAR) intracellular receptors. We provide genetic evidence that a previously uncharacterized member of this PP2C family in Arabidopsis (Arabidopsis thaliana), At5g59220, is a negative regulator of osmotic stress and ABA signaling and that this function was only apparent when double loss-of-function mutants with pp2ca-1/ahg3 were generated. At5g59220-green fluorescent protein and its close relative PP2CA-green fluorescent protein showed a predominant nuclear localization; however, hemagglutinin-tagged versions were also localized to cytosol and microsomal pellets. At5g59220 was selectively inhibited by some PYR/PYL ABA receptors, and close relatives of this PP2C, such as PP2CA/ABA-HYPERSENSITIVE GERMINATION3 (AHG3) and AHG1, showed a contrasting sensitivity to PYR/PYL inhibition. Interestingly, AHG1 was resistant to inhibition by the PYR/PYL receptors tested, which suggests that this seed-specific phosphatase is still able to regulate ABA signaling in the presence of ABA and PYR/PYL receptors and therefore to control the highly active ABA signaling pathway that operates during seed development. Moreover, the differential sensitivity of the phosphatases At5g59220 and PP2CA to inhibition by ABA receptors reveals a functional specialization of PYR/PYL ABA receptors to preferentially inhibit certain PP2Cs.
Framework for Intelligent Swimming Analytics with Wearable Sensors for Stroke Classification
Publication . Costa, Joana; Silva, Catarina; Santos, Miguel; Fernandes, Telmo; Faria, Sérgio
Intelligent approaches in sports using IoT devices to gather data, attempting to optimize athlete’s training and performance, are cutting edge research. Synergies between recent wearable hardware and wireless communication strategies, together with the advances in intelligent algorithms, which are able to perform online pattern recognition and classification with seamless results, are at the front line of high-performance sports coaching. In this work, an intelligent data analytics system for swimmer performance is proposed. The system includes (i) pre-processing of raw signals; (ii) feature representation of wearable sensors and biosensors; (iii) online recognition of the swimming style and turns; and (iv) post-analysis of the performance for coaching decision support, including stroke counting and average speed. The system is supported by wearable inertial (AHRS) and biosensors (heart rate and pulse oximetry) placed on a swimmer’s body. Radio-frequency links are employed to communicate with the heart rate sensor and the station in the vicinity of the swimming pool, where analytics is carried out. Experiments were carried out in a real training setup, including 10 athletes aged 15 to 17 years. This scenario resulted in a set of circa 8000 samples. The experimental results show that the proposed system for intelligent swimming analytics with wearable sensors effectively yields immediate feedback to coaches and swimmers based on real-time data analysis. The best result was achieved with a Random Forest classifier with a macro-averaged F1 of 95.02%. The benefit of the proposed framework was demonstrated by effectively supporting coaches while monitoring the training of several swimmers.
Evaluation of Adolescent Swimmers Through a 30-s Tethered Test
Publication . Morouço, Pedro G.; Vilas-Boas, João P.; Fernandes, Ricardo J.
Our purpose was to demonstrate that 30-s tethered swimming test can be a useful tool to estimate swimming performance in short distance freestyle events. Thirteen high level adolescent swimmers (7 male and 6 female of16.6 ± 1.0 and 15.8 ± 0.8 years old) performed a 30-s maximum effort in front crawl tethered swimming. Afterward, subjects completed 50-m and 100-m freestyle events at the National Championships. Both maximum and mean force values obtained in the tethered test related directly with 50-m (r =.78 and r =.72, p <.01, respectively) and 100-m freestyle velocities (r =.63 and r =.61, p <.05, respectively). Fatigue index did not present a significant relationship with any of the studied performance variables. However, a proposed parameter-fatigue slope-correlated with 50-m (r=-.75, p <.01), 100-m performances (r=-.57, p <.05) and with r[La-] (r=-.90, p <.01). It is concluded that, for adolescent swimmers, values obtained from 30-s tethered test are well related with swimming performance in sprint events. In addition, fatigue slope seems to be more associated with swimming performance in short distance events than fatigue index.
Database Marketing Process Supported by Ontologies
Publication . Mota Pinto, Filipe; Marques, Alzira; Santos, Manuel Filipe
The dramatic explosion of data and the growing number of different data sources are exposing researchers to a new challenge - how to acquire, maintain, and share knowledge from large databases in the context of rapidly applied and evolving research. This paper describes research on an ontological approach for leveraging the semantic content of ontologies to improve knowledge discovery in databases. We analyze how ontologies and knowledge discovery process may interoperate and present our efforts to bridge the two fields, knowledge discovery in databases and ontology learning for successful database usage projects.
Influence of Blood Contamination on Push-Out Bond Strength of Three Calcium Silicate-Based Materials to Root Dentin
Publication . Paulo, Cristina Rodrigues; Marques, Joana A.; Sequeira, Diana B.; Diogo, Patrícia; Paiva, Rui; Palma, Paulo J.; Santos, João Miguel
A proper bond between root canal filling materials and dentin surface is essential to resist dislodgement and guarantee long-term success. Blood exposure is likely to occur in various clinical situations in which calcium silicate-based materials are used; therefore, it is fundamental to render data concerning the influence of blood on bond strength. The present study aims to evaluate the effect of blood contamination on the push-out bond strength obtained with three different biomaterials to root canal dentin; Ninety extracted human mono-radicular permanent teeth were selected. The root canals were prepared with Gates Glidden burs until a diameter of 1.10 mm was achieved. Teeth were then randomly divided into six experimental groups (n = 15) according to the presence/absence of blood contamination and biomaterial used for root canal filling (ProRoot® MTA, Biodentine™, and TotalFill® BC Putty). After one week, each root was sectioned in three segments (coronal, middle, and apical regions). Specimens were then submitted to push-out bond strength tests. Fracture pattern evaluation was performed. The significance level was set at 5%.; Blood contamination did not affect the push-out bond strength of any of the three tested calcium silicate-based cements (p > 0.05). Regardless of blood contamination, TotalFill showed statistically higher push-out bond strength when compared with Biodentine (p = 0.040) and MTA (p = 0.004). Biodentine exhibited higher bond strength than MTA (p = 0.043). Biomaterials’ comparison within each radicular segment revealed statistically superior bond strength of both Biodentine and TotalFill over MTA (p < 0.05) in the coronal segment. TotalFill presented higher push-out bond strength regarding the apical segment compared to Biodentine (p = 0.003). Fractures were mostly adhesive.; Overall results indicate TotalFill presents the highest push-out bond strength values, followed by Biodentine and, lastly, MTA. Blood contamination did not affect the dislodgement resistance. Biomaterials’ comparison within each radicular segment revealed both TotalFill and Biodentine as the preferable alternatives for application in the coronal region. TotalFill might be the biomaterial of choice for placement in the apical region.