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In Situ Forming Silk Sericin-Based Hydrogel: A Novel Wound Healing Biomaterial

2021-03-17Journal article Restricted access
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dc.contributor.authorBaptista-Silva, Sara
dc.contributor.authorBorges, Sandra
dc.contributor.authorCosta-Pinto, Ana Rita
dc.contributor.authorCosta, Raquel
dc.contributor.authorAmorim, Manuela
dc.contributor.authorDias, Juliana R.
dc.contributor.authorRamos, Óscar
dc.contributor.authorAlves, Paulo
dc.contributor.authorGranja, Pedro Lopes
dc.contributor.authorSoares, Raquel
dc.contributor.authorPintado, Manuela
dc.contributor.authorOliveira, Ana Leite
dc.date.accessioned2023-03-15T14:43:26Z
dc.date.available2023-03-15T14:43:26Z
dc.date.issued2021-03-17
dc.description.abstractIn situ cross-linked hydrogels have the advantage of effectively fulfilling the wound in its shape and depth. Amongst the new generation of natural-based biopolymers being proposed for wound care and skin regeneration, silk sericin is particularly interesting due to its exceptional properties such as biocompatibility, biodegradability, and antioxidant behavior, among others. In this study, a new enzyme-mediated cross-linked hydrogel composed of silk sericin is proposed for the first time. The developed hydrogel crosslinking strategy was performed via horseradish peroxidase, under physiological conditions, and presented gelling kinetics under 3 min, as demonstrated by its rheological behavior. The hydrogels presented a high degree of transparency, mainly due to their amorphous conformation. Degradation studies revealed that the hydrogels were stable in phosphate buffer solution (PBS) (pH 7.4) for 17 days, while in the presence of protease XIV (3.5 U/ mg) and under acute and chronic physiological pH values, the stability decreased to 7 and 4 days, respectively. During protease degradation, the present sericin hydrogels demonstrated antioxidant activity. In vitro studies using an L929 fibroblast cell line demonstrated that these hydrogels were noncytotoxic, promoting cell adhesion and massive cell colonization after 7 days of culture, demonstrating that cells maintained their viability and proliferation. In addition, the application of sericin-based hydrogel in an in vivo diabetic wound model validated the feasibility of the in situ methodology and demonstrated a local anti-inflammatory effect, promoting the healing process. This study presents a simple, fast, and practical in situ approach to produce a sericin-based hydrogel able to be applied in low exudative chronic wounds. Moreover, the study herein reported fosters the valorization of a textile industrial by-product by its integration in the biomedical field.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationIn Situ Forming Silk Sericin-Based Hydrogel: A Novel Wound Healing Biomaterial Sara Baptista-Silva, Sandra Borges, Ana Rita Costa-Pinto, Raquel Costa, Manuela Amorim, Juliana R. Dias, Óscar Ramos, Paulo Alves, Pedro Lopes Granja, Raquel Soares, Manuela Pintado, and Ana Leite Oliveira ACS Biomaterials Science & Engineering 2021 7 (4), 1573-1586 DOI: 10.1021/acsbiomaterials.0c01745pt_PT
dc.identifier.doi10.1021/acsbiomaterials.0c01745pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.8/8225
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherACS Publicationspt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectPeptides and proteinspt_PT
dc.subjectHydrogelspt_PT
dc.subjectFiberspt_PT
dc.subjectDegradationpt_PT
dc.subjectBiomimetic materialspt_PT
dc.titleIn Situ Forming Silk Sericin-Based Hydrogel: A Novel Wound Healing Biomaterialpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage1586pt_PT
oaire.citation.startPage1573pt_PT
oaire.citation.titleACS Biomaterpt_PT
oaire.citation.volumeVol. 7, n.º 4pt_PT
person.familyNameDias
person.givenNameJuliana
person.identifier633669
person.identifier.ciencia-id0612-D971-F00D
person.identifier.orcid0000-0002-7079-1069
person.identifier.ridA-6086-2014
person.identifier.scopus-author-id55749822200
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication9e70a657-1b71-4366-ab59-b9f988a19d5a
relation.isAuthorOfPublication.latestForDiscovery9e70a657-1b71-4366-ab59-b9f988a19d5a

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