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Chondrogenic differentiation of mesenchymal stem/stromal cells on 3D porous poly (ε-caprolactone) scaffolds: Effects of material alkaline treatment and chondroitin sulfate supplementation

2020-06Journal article Restricted access
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dc.contributor.authorMoura, Carla
dc.contributor.authorSilva, João Carlos
dc.contributor.authorFaria, Sofia
dc.contributor.authorFernandes, Paulo Rui
dc.contributor.authorSilva, Cláudia Lobato da
dc.contributor.authorCabral, Joaquim Manuel Sampaio
dc.contributor.authorLinhardt, Robert
dc.contributor.authorBártolo, Paulo Jorge
dc.contributor.authorFerreira, Frederico Castelo
dc.date.accessioned2023-04-19T10:55:26Z
dc.date.available2023-04-19T10:55:26Z
dc.date.issued2020-06
dc.descriptionThis work was supported by FCT - Portuguese Foundation for Science and Technology through the projects UID/BIO/04565/2020, UID/Multi/04044/2019, UIDB/500022/2020, PTDC/BBB-BMC/5655/ 2014, and PRECISE e Accelerating progress toward the new era of precision medicine (PAC-PRECISE-LISBOA-01-0145- FEDER e 016394) and Stimuli2BioScaffold (FCT grant PTDC/EMESIS/ 32554/2017). Funding received from Programa Operacional Regional de Lisboa 2020 (Project No. 007317), MIT Portugal Program and through project POCI 01-0145-FEDER-016800 is also acknowledged. Carla Sofia Moura and João Carlos Silva are also thankful to FCT for funding received through the scholarships SFRH/BD/73970/2010 and SFRH/BD/105771/2014, respectively.pt_PT
dc.description.abstractCartilage defects resultant from trauma or degenerative diseases (e.g., osteoarthritis) can potentially be repaired using tissue engineering (TE) strategies combining progenitor cells, biomaterial scaffolds and bio physical/chemical cues. This work examines promoting chondrogenic differentiation of human bone marrow mesenchymal stem/stromal cells (BMMSCs) by combining the effects of modified poly (ε-caprolactone) (PCL) scaffolds hydrophilicity and chondroitin sulfate (CS) supplementation in a hypoxic 5% oxygen atmosphere. 3D extruded PCL scaffolds, characterized by mCT, featured a 21 mmL1 surface area to volume ratio, 390 mm pore size and approximately 100% pore interconnectivity. Scaffold immersion in sodium hydroxide solutions for different periods of time had major effects in scaffold surface morphology, wettability and mechanical properties, but without improvements on cell adhesion. In-situ chondrogenic differentiation of BM-MSC seeded in 3D-extruded PCL scaffolds resulted in higher cell populations and ECM deposition along all scaffold structure, when chondrogenesis was preceded by an expansion phase. Additionally, CS supplementation during BM-MSC expansion was crucial to enhance aggrecan gene expression, known as a hallmark of chondrogenesis. Overall, this study presents an approach to tailor the wettability and mechanical properties of PCL scaffolds and supports the use of CSsupplementation as a biochemical cue in integrated TE strategies for cartilage regeneration.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationMoura, C. S., Silva, J. C., Faria, S., Fernandes, P. R., da Silva, C. L., Cabral, J. M. S., Linhardt, R., Bártolo, P. J., & Ferreira, F. C. (2020). Chondrogenic differentiation of mesenchymal stem/stromal cells on 3D porous poly (ε-caprolactone) scaffolds: Effects of material alkaline treatment and chondroitin sulfate supplementation. Journal of Bioscience and Bioengineering, 129(6), 756–764. https://doi.org/10.1016/j.jbiosc.2020.01.004pt_PT
dc.identifier.doi10.1016/j.jbiosc.2020.01.004pt_PT
dc.identifier.issn1389-1723
dc.identifier.urihttp://hdl.handle.net/10400.8/8414
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationCentre for Rapid and Sustainable Product Development
dc.relationDESIGN AND FABRICATION OF ZONAL CARTILAGE CONSTRUCTS
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectArticular cartilage tissue engineeringpt_PT
dc.subjectBone marrow mesenchymal stem/stromal cellspt_PT
dc.subjectChondroitin sulfatept_PT
dc.subjectFused deposition modelingpt_PT
dc.subjectHypoxiapt_PT
dc.subjectPoly (ε-caprolactone) scaffoldspt_PT
dc.titleChondrogenic differentiation of mesenchymal stem/stromal cells on 3D porous poly (ε-caprolactone) scaffolds: Effects of material alkaline treatment and chondroitin sulfate supplementationpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleCentre for Rapid and Sustainable Product Development
oaire.awardTitleDESIGN AND FABRICATION OF ZONAL CARTILAGE CONSTRUCTS
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FMulti%2F04044%2F2019/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F73970%2F2010/PT
oaire.citation.endPage764pt_PT
oaire.citation.issue6pt_PT
oaire.citation.startPage756pt_PT
oaire.citation.titleJournal of Bioscience and Bioengineeringpt_PT
oaire.citation.volume129pt_PT
oaire.fundingStream6817 - DCRRNI ID
person.familyNameMonteiro de Moura
person.givenNameCarla Sofia
person.identifier.ciencia-id671C-8FEC-A14A
person.identifier.orcid0000-0003-2610-1005
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
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relation.isAuthorOfPublication.latestForDiscovery33d084bb-ac8d-454a-ac58-25be1331ce04
relation.isProjectOfPublicationaba3abaa-39ca-424c-a7d7-4d06649c5236
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