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Effects of Different Fibre Alignments and Bioactive Coatings on Mesenchymal Stem/Stromal Cell Adhesion and Proliferation in Poly (ɛ-caprolactone) Scaffolds towards Cartilage Repair

2017Journal article Open access
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datacite.subject.sdg03:Saúde de Qualidade
datacite.subject.sdg09:Indústria, Inovação e Infraestruturas
datacite.subject.sdg12:Produção e Consumo Sustentáveis
dc.contributor.authorSilva, João Carlos
dc.contributor.authorMoura, Carla Sofia
dc.contributor.authorAlves, Nuno
dc.contributor.authorCabral, Joaquim Manuel Sampaio
dc.contributor.authorFerreira, Frederico Castelo
dc.date.accessioned2025-12-15T16:28:55Z
dc.date.available2025-12-15T16:28:55Z
dc.date.issued2017
dc.description.abstractIn this work, 3D biodegradable poly (ɛ-caprolactone) scaffolds with high porosity and interconnectivity were produced by extrusion and characterized in terms of their structural and mechanical properties. The effects of scaffold fibre alignment (0-45° and 0-90°) and of different adhesive coatings (Fibronectin, Gelatin and commercially available CELLstart™) on bone marrow mesenchymal stem/stromal cells adhesion, migration and proliferation were evaluated using AlamarBlue™ assay. Fibronectin and CELLstart™ coated poly (ɛ-caprolactone) scaffolds presented an improvement in cell adhesion of approximately 2-fold relatively to the non-treated scaffolds, independently of fibre alignment. Overall, all the conditions studied promoted the growth and migration of bone marrow mesenchymal stem cells in the fabricated poly(ɛ-caprolactone) scaffolds, without affecting their chondrogenic differentiation and extracellular matrix deposition evaluated by Alcian Blue staining.eng
dc.description.sponsorshipThe authors gratefully acknowledge the financial support from FCT - Portuguese Foundation for Science and Technology through the scholarship SFRH/BD/105771/2014 and projects UID/BIO/04565/2013, UID/Multi/04044/2013. Funding received from Programa Operacional Regional de Lisboa 2020 (Project Nº 007317) and through the project POCI-01-0145-FEDER-016800 and POCI-01-0247-FEDER-017771 is also acknowledged.
dc.identifier.citationJoão Carlos Silva, Carla Sofia Moura, Nuno Alves, Joaquim Manuel Sampaio Cabral, Frederico Castelo Ferreira, Effects of Different Fibre Alignments and Bioactive Coatings on Mesenchymal Stem/Stromal Cell Adhesion and Proliferation in Poly (ɛ-caprolactone) Scaffolds towards Cartilage Repair, Procedia Manufacturing, Volume 12, 2017, Pages 132-140, ISSN 2351-9789, https://doi.org/10.1016/j.promfg.2017.08.034
dc.identifier.doi10.1016/j.promfg.2017.08.034
dc.identifier.issn2351-9789
dc.identifier.urihttp://hdl.handle.net/10400.8/15065
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier BV
dc.relationSFRH/BD/105771/2014
dc.relationUID/BIO/04565/2013
dc.relation.hasversionhttps://www.sciencedirect.com/science/article/pii/S2351978917306236
dc.relation.ispartofProcedia Manufacturing
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectBiomanufacturingCartilage Tissue EngineeringCell AdhesionMesenchymal Stem/Stromal CellsPoly (ɛ-caprolactone) Scaffolds
dc.subjectCartilage Tissue Engineering
dc.subjectCell Adhesion
dc.subjectMesenchymal Stem/Stromal Cells
dc.subjectPoly (ɛ-caprolactone) Scaffolds
dc.titleEffects of Different Fibre Alignments and Bioactive Coatings on Mesenchymal Stem/Stromal Cell Adhesion and Proliferation in Poly (ɛ-caprolactone) Scaffolds towards Cartilage Repaireng
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage140
oaire.citation.startPage132
oaire.citation.volume12
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameMonteiro de Moura
person.familyNameAlves
person.givenNameCarla Sofia
person.givenNameNuno
person.identifier452149
person.identifier.ciencia-id671C-8FEC-A14A
person.identifier.ciencia-id311E-1559-8F6C
person.identifier.orcid0000-0003-2610-1005
person.identifier.orcid0000-0002-5016-0868
person.identifier.ridN-4073-2013
person.identifier.scopus-author-id7006403383
relation.isAuthorOfPublication33d084bb-ac8d-454a-ac58-25be1331ce04
relation.isAuthorOfPublicationbbd46a74-b77e-4539-a5fe-62ee95cdc4fa
relation.isAuthorOfPublication.latestForDiscovery33d084bb-ac8d-454a-ac58-25be1331ce04

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