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Effects of Human Mesenchymal Stem Cells Isolated from Wharton’s Jelly of the Umbilical Cord and Conditioned Media on Skeletal Muscle Regeneration Using a Myectomy Model

2014-10-14Artigo científico Acesso aberto
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datacite.subject.fosCiências Médicas::Ciências da Saúde
datacite.subject.sdg03:Saúde de Qualidade
dc.contributor.authorPereira, T.
dc.contributor.authorArmada-da Silva, P.A.S.
dc.contributor.authorAmorim, I.
dc.contributor.authorRêma, A.
dc.contributor.authorCaseiro, A.R.
dc.contributor.authorGärtner, A.
dc.contributor.authorRodrigues, M.
dc.contributor.authorLopes, M. A.
dc.contributor.authorBartolo, P.J.
dc.contributor.authorSantos, J.D.
dc.contributor.authorLuís, A.L.
dc.contributor.authorMaurício, A.C.
dc.date.accessioned2026-05-06T12:37:23Z
dc.date.available2026-05-06T12:37:23Z
dc.date.issued2014-10-14
dc.descriptionArticle - 376918
dc.description.abstractkeletal muscle has good regenerative capacity, but the extent of muscle injury and the developed fibrosis might prevent complete regeneration. The<jats:italic>in vivo</jats:italic>application of human mesenchymal stem cells (HMSCs) of the umbilical cord and the conditioned media (CM) where the HMSCs were cultured and expanded, associated with different vehicles to induce muscle regeneration, was evaluated in a rat myectomy model. Two commercially available vehicles and a spherical hydrogel developed by our research group were used. The treated groups obtained interesting results in terms of muscle regeneration, both in the histological and in the functional assessments. A less evident scar tissue, demonstrated by collagen type I quantification, was present in the muscles treated with HMSCs or their CM. In terms of the histological evaluation performed by ISO 10993-6 scoring, it was observed that HMSCs apparently have a long-term negative effect, since the groups treated with CM presented better scores. CM could be considered an alternative to the<jats:italic>in vivo</jats:italic>transplantation of these cells, as it can benefit from the local tissue response to secreted molecules with similar results in terms of muscular regeneration. Searching for an optimal vehicle might be the key point in the future of skeletal muscle tissue engineering.eng
dc.description.sponsorshipThis research was supported by QREN I&DT Cluster in Development of Products for Regenerative Medicine and Cell Therapies-Projects Biomat & Cell QREN 2008/1372, cofinanced by the European Community FEDER fund through ON2-O Novo Norte-North Portugal Regional Operational Program 2007-2013, by QREN number 38853/ 2013-"Soluções avançadas de regeneração óssea com base em hidrogéis de dextrin-DEXGELERATION," by project "Hybrid Nanostructured Hydrogels: Bone regeneration using Multifunctional injectable Hydrogels-Rebone"- ENMED/0002/2010, and by the program COMPETE- Programa Operacional Factores de Competitividade, Project Pest-OE/AGR/UI0211/2011. I. Amorim (SFRH/BD/ 76237/2011) acknowledges FCT, the Portuguese Foundation for Science and Technology, for financial support.
dc.description.sponsorshipIPATIMUP is partially supported by FCT.
dc.identifier.citationPereira, T., Armada-da Silva, P. A. S., Amorim, I., Rêma, A., Caseiro, A. R., Gärtner, A., Rodrigues, M., Lopes, M. A., Bártolo, P. J., Santos, J. D., Luís, A. L., & Maurício, A. C. (2014). Effects of Human Mesenchymal Stem Cells Isolated from Wharton’s Jelly of the Umbilical Cord and Conditioned Media on Skeletal Muscle Regeneration Using a Myectomy Model. Stem Cells International, 2014, Article 376918. https://doi.org/10.1155/2014/376918
dc.identifier.doi10.1155/2014/376918
dc.identifier.issn1687-966X
dc.identifier.issn1687-9678
dc.identifier.urihttp://hdl.handle.net/10400.8/16238
dc.language.isoeng
dc.peerreviewedyes
dc.publisherWiley
dc.relationHybrid Nanostuctured Hydrogels: Bone regeneration using multifunctional injectable hydrogels
dc.relationStrategic Project - UI 211 - 2011-2012
dc.relationHELICOBACTER SPP. INFECTION IN PETS - AN EPIDEMIOLOGICAL, MOLECULAR AND PREVENTIVE STUDY
dc.relation.hasversionhttps://onlinelibrary.wiley.com/doi/10.1155/2014/376918
dc.relation.ispartofStem Cells International
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectMesenchymal stem cells
dc.subjectWharton’s jelly
dc.subjectumbilical cord
dc.subjectconditioned media
dc.subjectmuscle regeneration
dc.subjectmyectomy model
dc.subjectrat
dc.subjectfibrin glue
dc.subjectFloseal
dc.subjecthydrogel
dc.titleEffects of Human Mesenchymal Stem Cells Isolated from Wharton’s Jelly of the Umbilical Cord and Conditioned Media on Skeletal Muscle Regeneration Using a Myectomy Modeleng
dc.typejournal article
dspace.entity.typePublication
oaire.awardNumberENMED/0002/2010
oaire.awardNumberPEst-OE/AGR/UI0211/2011
oaire.awardNumberSFRH/BD/76237/2011
oaire.awardTitleHybrid Nanostuctured Hydrogels: Bone regeneration using multifunctional injectable hydrogels
oaire.awardTitleStrategic Project - UI 211 - 2011-2012
oaire.awardTitleHELICOBACTER SPP. INFECTION IN PETS - AN EPIDEMIOLOGICAL, MOLECULAR AND PREVENTIVE STUDY
oaire.awardURIhttp://hdl.handle.net/10400.8/16236
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/PEst-OE%2FAGR%2FUI0211%2F2011/PT
oaire.awardURIhttp://hdl.handle.net/10400.8/16237
oaire.citation.endPage16
oaire.citation.startPage1
oaire.citation.titleStem Cells International
oaire.citation.volume2014
oaire.fundingStreamEuroNanoMed - Second Joint Call 2010
oaire.fundingStream6817 - DCRRNI ID
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameCaseiro Santos
person.familyNameBartolo
person.givenNameAna Rita
person.givenNamePaulo
person.identifier203086
person.identifier.ciencia-id5810-9BF9-4522
person.identifier.orcid0000-0002-1143-2461
person.identifier.orcid0000-0003-3683-726X
person.identifier.ridF-2421-2013
person.identifier.scopus-author-id6603353041
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
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