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Development of novel 3D scaffolds using BioExtruder by varying the content of hydroxyapatite and silica in PCL matrix for bone tissue engineering

2020-03-07Journal article Restricted access
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dc.contributor.authorPattanashetti, Nandini A.
dc.contributor.authorViana, Tânia
dc.contributor.authorAlves, Nuno
dc.contributor.authorMitchell, Geoffrey
dc.contributor.authorKariduraganavar, Mahadevappa Y.
dc.date.accessioned2023-04-19T15:15:58Z
dc.date.available2023-04-19T15:15:58Z
dc.date.issued2020-03-07
dc.descriptionAuthors wish to thank the DST, New Delhi, for extending the financial support under DST-PURSE-Phase-II Program (Grant No. SR/PURSE-Phase-2/3-G). Authors will remain grateful to Skanda Life Sciences Pvt. Ltd., Bangalore, for supporting the cell culture studypt_PT
dc.description.abstractPolycaprolactone (PCL) is considered as a most widely used biodegradable polymers in tissue engineering. But, PCL is also associated with certain limitations like, low stiffness, hydrophobic nature and limited cell affinity. These drawbacks are addressed in the present study by incorporating different wt% of silicon dioxide (SiO2) and hydroxyapatite (HAp) in the PCL matrix. 3D scaffolds were developed using a novel BioExtruder. The physicochemical properties, thermal stability and wettability of the composite scaffolds were studied systematically. Optical and Scanning Electron Microscopic images were analysed for morphological evaluation of the scaffolds. The pore size of the developed scaffolds increased from 290 to 315 μmwith increasing SiO2 content, as examined by scanning electron microscope. An improved compressive modulus of 68.82 MPa was observed for 15 wt% SiO2 incorporated composite scaffold. The in-vitro degradation study of the composite scaffolds demonstrated an increase in the degradation rate for PCL/HAp scaffolds, while no significant change was observed for SiO2 incorporated scaffolds. Further, the cytotoxicity and cell proliferation studies were carried out using L929 Mouse Fibroblasts and MG-63 Osteoblasts respectively. The developed scaffolds revealed no toxic effects towards the cellular response and an increase in cell proliferation of ≥90% was observed during 7 days of cell culture. Thus, the scaffolds were proved to be potential candidate for bone tissue engineering application, particularly the scaffold with 10 wt% SiO2 incorporation into PCL/HAp (75/15) composite has resulted into higher cell proliferative % and improved mechanical strength.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationPattanashetti, N. A., Viana, T., Alves, N., Mitchell, G. R., & Kariduraganavar, M. Y. (2020). Development of novel 3D scaffolds using BioExtruder by varying the content of hydroxyapatite and silica in PCL matrix for bone tissue engineering. Journal of Polymer Research, 27(87). https://doi.org/10.1007/s10965-020-02053-0pt_PT
dc.identifier.doi10.1007/s10965-020-02053-0pt_PT
dc.identifier.issn1022-9760
dc.identifier.issn1572-8935
dc.identifier.urihttp://hdl.handle.net/10400.8/8422
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringerpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectBiodegradablept_PT
dc.subjectScaffoldpt_PT
dc.subjectBone tissue engineeringpt_PT
dc.subjectThree dimensionalpt_PT
dc.subjectWettabilitypt_PT
dc.subjectBiocompatibilitypt_PT
dc.titleDevelopment of novel 3D scaffolds using BioExtruder by varying the content of hydroxyapatite and silica in PCL matrix for bone tissue engineeringpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue87pt_PT
oaire.citation.titleJournal of Polymer Researchpt_PT
oaire.citation.volume27pt_PT
person.familyNameViana
person.familyNameAlves
person.familyNameMitchell
person.givenNameTânia
person.givenNameNuno
person.givenNameGeoffrey
person.identifier452149
person.identifier166356
person.identifier.ciencia-idB41D-8BE0-7C9D
person.identifier.ciencia-id311E-1559-8F6C
person.identifier.ciencia-idE41A-ABDD-1FC7
person.identifier.orcid0000-0002-9688-647X
person.identifier.orcid0000-0002-5016-0868
person.identifier.orcid0000-0001-7977-7610
person.identifier.ridO-1147-2013
person.identifier.ridN-4073-2013
person.identifier.scopus-author-id55885892100
person.identifier.scopus-author-id7006403383
person.identifier.scopus-author-id7403103397
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
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relation.isAuthorOfPublication48c8066b-023e-4405-b462-49d28af000d1
relation.isAuthorOfPublication.latestForDiscoveryfe6d67b5-016d-4953-a5eb-9e357af79793

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