Loading...
Publication
Fast decellularization process using supercritical carbon dioxide for trabecular bone
| Name: | Description: | Size: | Format: | |
|---|---|---|---|---|
| 8.28 MB | Adobe PDF |
Advisor(s)
Abstract(s)
Decellularization is a process that consists on the removal of immunogenic cellular material from a tissue, so that
it can be safely implanted as a functional and bioactive scaffold. Most decellularization protocols rely on the use
of harsh chemicals and very long washing processes, leading to severe changes in the ultrastructure and loss of
mechanical integrity. To tackle these challenges, supercritical carbon dioxide (scCO2) is herein proposed as an
alternative methodology for assisting decellularization of porcine trabecular bone tissue and is combined, for the
first time, with Tri(n-butyl) phosphate (TnBP). Histological and DNA analysis revealed that both TnBP and scCO2
were able to extract the DNA content from the scaffolds, being this effect more pronounced in treatments that
used TnBP as a co-solvent. The combined protocol led to a decrease in DNA content by at least 90%, demon-
strating the potential of this methodology and opening new possibilities for future optimizations.
Description
Keywords
Trabecular bone TnBP Supercritical carbon dioxide Decellularization
Citation
Marta M. Duarte, Nilza Ribeiro, Inês V. Silva, Juliana R. Dias, Nuno M. Alves, Ana L. Oliveira, Fast decellularization process using supercritical carbon dioxide for trabecular bone, The Journal of Supercritical Fluids, Volume 172, 2021, 105194, ISSN 0896-8446, https://doi.org/10.1016/j.supflu.2021.105194Duarte, Nilza Ribeiro, Inês V. Silva, Juliana R. Dias, Nuno M. Alves, Ana L. Oliveira, Fast decellularization process using supercritical carbon dioxide for trabecular bone, The Journal of Supercritical Fluids, Volume 172, 2021, 105194, ISSN 0896-8446, https://doi.org/10.1016/j.supflu.2021.105194
Publisher
Elsevier