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Extracellular matrix decorated polycaprolactone scaffolds for improved mesenchymal stem/stromal cell osteogenesis towards a patient-tailored bone tissue engineering approach

2020-01-09Journal article Restricted access
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dc.contributor.authorSilva, João C.
dc.contributor.authorCarvalho, Marta S.
dc.contributor.authorUdangawa, Ranodhi N.
dc.contributor.authorMoura, Carla
dc.contributor.authorCabral, Joaquim M. S.
dc.contributor.authorSilva, Cláudia L. da
dc.contributor.authorFerreira, Frederico Castelo
dc.contributor.authorVashishth, Deepak
dc.contributor.authorLinhardt, Robert J.
dc.date.accessioned2023-04-19T10:32:40Z
dc.date.available2023-04-19T10:32:40Z
dc.date.issued2020-01-09
dc.descriptionJ.C.S. and M.S.C. are grateful to Fundação para a Ciência e Tecnologia (FCT, Portugal) for financial support through the scholarships SFRH/ BD/105771/2014 and SFRH/BD/52478/2014, respectively. The authors acknowledge financial support from FCT through iBB— Institute for Bioengineering and Biosciences (UID/BIO/04565/2019) and from Programa Operacional Regional de Lisboa 2020 (Project N. 007317) and also through the projects PRECISE (PAC-PRECISELISBOA- 01-0145-FEDER-016394) and Stimuli2BioScaffold (FCT grant PTDC/EME-SIS/32554/2017). This study was also supported by Center for Biotechnology and Interdisciplinary Studies-Rensselaer Polytechnic Institute funds and by the National Institutes of Health (Grant # DK111958).pt_PT
dc.description.abstractThe clinical demand for tissue-engineered bone is growing due to the increase of non-union fractures and delayed healing in an aging population. Herein, we present a method combining additive manufacturing (AM) techniques with cell-derived extracellular matrix (ECM) to generate structurally well-defined bioactive scaffolds for bone tissue engineering (BTE). In this work, highly porous three-dimensional polycaprolactone (PCL) scaffolds with desired size and architecture were fabricated by fused deposition modeling and subsequently decorated with human mesenchymal stem/stromal cell (MSC)-derived ECM produced in situ. The successful deposition of MSC-derived ECM onto PCL scaffolds (PCL-MSC ECM) was confirmed after decellularization using scanning electron microscopy, elemental analysis, and immunofluorescence. The presence of cell derived ECM within the PCL scaffolds significantly enhanced MSC attachment and proliferation, with and without osteogenic supplementation. Additionally, under osteogenic induction, PCL-MSC ECM scaffolds promoted significantly higher calcium deposition and elevated relative expression of bone-specific genes, particularly the gene encoding osteopontin, when compared to pristine scaffolds. Overall, our results demonstrated the favorable effects of combining MSC-derived ECM and AM-based scaffolds on the osteogenic differentiation of MSC, resulting from a closer mimicry of the native bone niche. This strategy is highly promising for the development of novel personalized BTE approaches enabling the fabrication of patient defect-tailored scaffolds with enhanced biological performance and osteoinductive properties.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationSilva, J. C., Carvalho, M. S., Udangawa, R. N., Moura, C. S., Cabral, J. M. S., L. da Silva, C., Ferreira, F. C., Vashishth, D., & Linhardt, R. J. (2020). Extracellular matrix decorated polycaprolactone scaffolds for improved mesenchymal stem/stromal cell osteogenesis towards a patient-tailored bone tissue engineering approach. Journal of Biomedical Materials Research - Part B Applied Biomaterials, 108(5), 2153–2166. https://doi.org/10.1002/jbm.b.34554pt_PT
dc.identifier.doi10.1002/jbm.b.34554pt_PT
dc.identifier.issn1552-4973
dc.identifier.issn1552-4981
dc.identifier.urihttp://hdl.handle.net/10400.8/8412
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherWileypt_PT
dc.relationInstitute for Bioengineering and Biosciences
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAdditive manufacturingpt_PT
dc.subjectBone tissue engineeringpt_PT
dc.subjectCell-derived extracellular matrixpt_PT
dc.subjectMesenchymal stem/stromal cellspt_PT
dc.subjectPolycaprolactone scaffoldspt_PT
dc.titleExtracellular matrix decorated polycaprolactone scaffolds for improved mesenchymal stem/stromal cell osteogenesis towards a patient-tailored bone tissue engineering approachpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleInstitute for Bioengineering and Biosciences
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FBIO%2F04565%2F2019/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/9471 - RIDTI/PTDC%2FEME-SIS%2F32554%2F2017/PT
oaire.citation.endPage2166pt_PT
oaire.citation.issue5pt_PT
oaire.citation.startPage2153pt_PT
oaire.citation.titleJournal of Biomedical Materials Research Part B: Applied Biomaterialspt_PT
oaire.citation.volume108pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream9471 - RIDTI
person.familyNameMonteiro de Moura
person.givenNameCarla Sofia
person.identifier.ciencia-id671C-8FEC-A14A
person.identifier.orcid0000-0003-2610-1005
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication33d084bb-ac8d-454a-ac58-25be1331ce04
relation.isAuthorOfPublication.latestForDiscovery33d084bb-ac8d-454a-ac58-25be1331ce04
relation.isProjectOfPublication84f0e711-a0c5-49df-9af7-f7b2498375f7
relation.isProjectOfPublication332a09b1-c6c0-4c3d-9e39-0195b5047243
relation.isProjectOfPublication.latestForDiscovery84f0e711-a0c5-49df-9af7-f7b2498375f7

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