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Extruded bioreactor perfusion culture supports the chondrogenic differentiation of human mesenchymal stem/stromal cells in 3D porous poly(ɛ-caprolactone) scaffolds

2020-02Journal article Restricted access
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dc.contributor.authorSilva, João C.
dc.contributor.authorMoura, Carla
dc.contributor.authorBorrecho, Gonçalo
dc.contributor.authorMatos, António P. Alves de
dc.contributor.authorSilva, Cláudia L. da
dc.contributor.authorCabral, Joaquim M. S.
dc.contributor.authorBártolo, Paulo J.
dc.contributor.authorLinhardt, Robert J.
dc.contributor.authorFerreira, Frederico Castelo
dc.date.accessioned2023-04-17T15:20:43Z
dc.date.available2023-04-17T15:20:43Z
dc.date.issued2020-02
dc.descriptionThe authors acknowledge financial support from Fundação para a Ciência e Tecnologia (FCT, Portugal) through UID/BIO/04565/2019, UID/Multi/04044/2013), Stimuli2BioScaffold grant (PTDC/EMESIS/ 32554/2017), and scholarships SFRH/BD/73970/2010, SFRH/BD/ 105771/2014, and SFRH/BSAB/128442/2017. Funding from Programa Operacional Regional de Lisboa 2020 (project no. 007317) and also through the projects PRECISE (PAC-PRECISE-LISBOA-01-0145-FEDER – 016394, SAICTPAC/0021/2015) and POCI-01-0145 FEDER-016800 is also acknowledged.pt_PT
dc.description.abstractNovel bioengineering strategies for the ex vivo fabrication of native-like tissue-engineered cartilage are crucial for the translation of these approaches to clinically manage highly prevalent and debilitating joint diseases. Bioreactors that provide different biophysical stimuli have been used in tissue engineering approaches aimed at enhancing the quality of the cartilage tissue generated. However, such systems are often highly complex, expensive, and not very versatile. In the current study, a novel, cost-effective, and customizable perfusion bioreactor totally fabricated by additive manufacturing (AM) is proposed for the study of the effect of fluid flow on the chondrogenic differentiation of human bone-marrow mesenchymal stem/stromal cells (hBMSCs) in 3D porous poly(ɛ-caprolactone) (PCL) scaffolds. hBMSCs are first seeded and grown on PCL scaffolds and hBMSC–PCL constructs are then transferred to 3D-extruded bioreactors for continuous perfusion culture under chondrogenic inductive conditions. Perfused constructs show similar cell metabolic activity and significantly higher sulfated glycosaminoglycan production (≈1.8-fold) in comparison to their non-perfused counterparts. Importantly, perfusion bioreactor culture significantly promoted the expression of chondrogenic marker genes while downregulating hypertrophy. This work highlights the potential of customizable AM platforms for the development of novel personalized repair strategies and more reliable in vitro models with a wide range of applications.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationSilva, J. C., Moura, C. S., Borrecho, G., de Matos, A. P. A., da Silva, C. L., Cabral, J. M. S., Bártolo, P. J., Linhardt, R. J., & Ferreira, F. C. (2020). Extruded Bioreactor Perfusion Culture Supports the Chondrogenic Differentiation of Human Mesenchymal Stem/Stromal Cells in 3D Porous Poly(ɛ-Caprolactone) Scaffolds. Biotechnology Journal, 15(2). https://doi.org/10.1002/biot.201900078pt_PT
dc.identifier.doidoi.org/10.1002/biot.201900078pt_PT
dc.identifier.issn1860-6768
dc.identifier.issn1860-7314
dc.identifier.urihttp://hdl.handle.net/10400.8/8401
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherWileypt_PT
dc.relationInstitute for Bioengineering and Biosciences
dc.relationCentre for Rapid and Sustainable Product Development
dc.relationDESIGN AND FABRICATION OF ZONAL CARTILAGE CONSTRUCTS
dc.relationBioreactor and process system design
dc.relationAccelerating progress toward the new era of precision medicine
dc.relation.publisherversionhttps://onlinelibrary.wiley.com/doi/full/10.1002/biot.201900078pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAdditive manufacturingpt_PT
dc.subjectCartilage tissue engineeringpt_PT
dc.subjectExtrusion-based perfusion bioreactorpt_PT
dc.subjectMesenchymal stem/stromal cellspt_PT
dc.subjectPoly(ɛ-caprolactone) scaffoldspt_PT
dc.titleExtruded bioreactor perfusion culture supports the chondrogenic differentiation of human mesenchymal stem/stromal cells in 3D porous poly(ɛ-caprolactone) scaffoldspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleInstitute for Bioengineering and Biosciences
oaire.awardTitleCentre for Rapid and Sustainable Product Development
oaire.awardTitleDESIGN AND FABRICATION OF ZONAL CARTILAGE CONSTRUCTS
oaire.awardTitleBioreactor and process system design
oaire.awardTitleAccelerating progress toward the new era of precision medicine
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FBIO%2F04565%2F2019/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FMulti%2F04044%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F73970%2F2010/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/SFRH%2FBSAB%2F128442%2F2017/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/9471 - RIDTI/SAICTPAC%2F0021%2F2015/PT
oaire.citation.issue2pt_PT
oaire.citation.titleBiotechnology Journalpt_PT
oaire.citation.volume15pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamOE
oaire.fundingStream9471 - RIDTI
person.familyNameSilva
person.familyNameMonteiro de Moura
person.givenNameJoão Carlos
person.givenNameCarla Sofia
person.identifier1715750
person.identifier.ciencia-id7F1B-FF18-FBAA
person.identifier.ciencia-id671C-8FEC-A14A
person.identifier.orcid0000-0003-4773-6771
person.identifier.orcid0000-0003-2610-1005
person.identifier.scopus-author-id57214120948
project.funder.identifierhttp://doi.org/10.13039/501100001871
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project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
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