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The first systematic analysis of 3D rapid prototyped poly(ε-caprolactone) scaffolds manufactured through BioCell printing: the effect of pore size and geometry on compressive mechanical behaviour and<i>in vitro</i>hMSC viability

2013-11-06Artigo científico Acesso restrito
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datacite.subject.fosEngenharia e Tecnologia::Biotecnologia Industrial
datacite.subject.sdg03:Saúde de Qualidade
datacite.subject.sdg09:Indústria, Inovação e Infraestruturas
datacite.subject.sdg12:Produção e Consumo Sustentáveis
dc.contributor.authorDomingos, M.
dc.contributor.authorIntranuovo, F.
dc.contributor.authorRusso, T.
dc.contributor.authorDe Santis, R.
dc.contributor.authorGloria, A.
dc.contributor.authorAmbrosio, L.
dc.contributor.authorCiurana, J.
dc.contributor.authorBártolo, P.
dc.date.accessioned2026-04-08T11:38:04Z
dc.date.available2026-04-08T11:38:04Z
dc.date.issued2013-11-06
dc.description.abstractNovel additive manufacturing processes are increasingly recognized as ideal techniques to produce 3D biodegradable structures with optimal pore size and spatial distribution, providing an adequate mechanical support for tissue regeneration while shaping in-growing tissues. With regard to the mechanical and biological performances of 3D scaffolds, pore size and geometry play a crucial role. In this study, a novel integrated automated system for the production and in vitro culture of 3D constructs, known as BioCell Printing, was used only to manufacture poly(ε-caprolactone) scaffolds for tissue engineering; the influence of pore size and shape on their mechanical and biological performances was investigated. Imposing a single lay-down pattern of 0°/90° and varying the filament distance, it was possible to produce scaffolds with square interconnected pores with channel sizes falling in the range of 245–433 µm, porosity 49–57% and a constant road width. Three different lay-down patterns were also adopted (0°/90°, 0°/60/120° and 0°/45°/90°/135°), thus resulting in scaffolds with quadrangular, triangular and complex internal geometries, respectively. Mechanical compression tests revealed a decrease of scaffold stiffness with the increasing porosity and number of deposition angles (from 0°/90° to 0°/45°/90°/135°). Results from biological analysis, carried out using human mesenchymal stem cells, suggest a strong influence of pore size and geometry on cell viability. On the other hand, after 21 days of in vitro static culture, it was not possible to detect any significant variation in terms of cell morphology promoted by scaffold topology. As a first systematic analysis, the obtained results clearly demonstrate the potential of the BioCell Printing process to produce 3D scaffolds with reproducible well organized architectures and tailored mechanical properties.eng
dc.description.sponsorshipThis work was performed with the partial financial support provided by the Fundação para a Ciência e Tecnologia through the strategic project Pest-OE/EME/UI4044/2011. Authors wish to thank Professor Geoffrey Mitchell and his team at CFAMfor their support in recording SEM images of scaffolds.
dc.identifier.citationDomingos, M., Intranuovo, F., Russo, T., Santis, R. D., Gloria, A., Ambrosio, L., ... & Bartolo, P. (2013). The first systematic analysis of 3D rapid prototyped poly (ε-caprolactone) scaffolds manufactured through BioCell printing: the effect of pore size and geometry on compressive mechanical behaviour and in vitro hMSC viability. Biofabrication, 5(4), 045004.
dc.identifier.doi10.1088/1758-5082/5/4/045004
dc.identifier.issn1758-5082
dc.identifier.issn1758-5090
dc.identifier.urihttp://hdl.handle.net/10400.8/16072
dc.language.isoeng
dc.peerreviewedyes
dc.publisherIOP Publishing
dc.relationStrategic Project - UI 4044 - 2011-2012
dc.relation.ispartofBiofabrication
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectBiocompatible Materials
dc.subjectBioprinting
dc.subjectCell Adhesion
dc.subjectCell Proliferation
dc.subjectCell Survival
dc.titleThe first systematic analysis of 3D rapid prototyped poly(ε-caprolactone) scaffolds manufactured through BioCell printing: the effect of pore size and geometry on compressive mechanical behaviour and<i>in vitro</i>hMSC viabilityeng
dc.typejournal article
dspace.entity.typePublication
oaire.awardNumberPEst-OE/EME/UI4044/2011
oaire.awardTitleStrategic Project - UI 4044 - 2011-2012
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/PEst-OE%2FEME%2FUI4044%2F2011/PT
oaire.citation.endPage13
oaire.citation.issue4
oaire.citation.startPage1
oaire.citation.titleBiofabrication
oaire.citation.volume5
oaire.fundingStream6817 - DCRRNI ID
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameDomingos
person.familyNameBartolo
person.givenNameMarco
person.givenNamePaulo
person.identifier203086
person.identifier.ciencia-idB512-2D06-5897
person.identifier.ciencia-id5810-9BF9-4522
person.identifier.orcid0000-0002-6693-790X
person.identifier.orcid0000-0003-3683-726X
person.identifier.ridF-2421-2013
person.identifier.scopus-author-id6603353041
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
relation.isAuthorOfPublication1fb3bd4b-f499-4db6-93c1-3a671f116d07
relation.isAuthorOfPublicationab44d1ae-46d0-45c2-b19f-200024b5a990
relation.isAuthorOfPublication.latestForDiscovery1fb3bd4b-f499-4db6-93c1-3a671f116d07
relation.isProjectOfPublication44bd1c93-9ee1-4685-a408-c2baa61ffbcd
relation.isProjectOfPublication.latestForDiscovery44bd1c93-9ee1-4685-a408-c2baa61ffbcd

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