Repository logo
 
Publication

Effects of glycosaminoglycan supplementation in the chondrogenic differentiation of bone marrow- and synovial- derived mesenchymal stem/stromal cells on 3D-extruded poly (ε-caprolactone) scaffolds

2020-01-02Journal article Open access
Simple item page
dc.contributor.authorSilva, João C.
dc.contributor.authorMoura, Carla
dc.contributor.authorMatos, António P. Alves de
dc.contributor.authorCabral, Joaquim M. S.
dc.contributor.authorLinhardt, Robert J.
dc.contributor.authorFerreira, Frederico Castelo
dc.date.accessioned2023-03-15T14:15:31Z
dc.date.available2023-03-15T14:15:31Z
dc.date.issued2020-01-02
dc.description.abstractThe lack of effective and long-term treatments for articular cartilage defects has increased the interest for innovative tissue engineering strategies. Such approaches, combining cells, biomaterial matrices and external biochemical/physical cues, hold promise for generating fully functional cartilage tissue. Herein, this study aims at exploring the use of the major cartilage glycosaminoglycans (GAGs), chondroitin sulfate (CS) and hyaluronic acid (HA), as external biochemical cues to promote the chondrogenic differentiation of human bone marrow- and synovium-derived mesenchymal stem/stromal cells (hBMSC/hSMSC) on custom-made 3 D porous poly (ε-caprolactone) (PCL) scaffolds. The culture conditions, namely the chondrogenic medium and hypoxic environment (5% O2 tension), were firstly optimized by culturing hBMSCs on PCL scaffolds without GAG supplementation. For both MSC sources, GAG supplemented media, particularly with HA, promoted significantly cartilage-like extracellular matrix (ECM) production (higher sulfated GAG amounts) and chondrogenic gene expression. Remarkably, in contrast to tissues generated using hBMSCs, the hSMSC-based constructs showed decreased expression of hypertrophic marker COL X. Histological, immunohistochemical and transmission electron microscopy (TEM) analysis confirmed the presence of typical articular cartilage ECM components (GAGs, aggrecan, collagen fibers) in all the tissue constructs produced. Overall, our results highlight the potential of integrating GAG supplementation, hSMSCs and customizable 3 D scaffolds toward the fabrication of bioengineered cartilage tissue substitutes with reduced hypertrophy.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationoão C. Silva, Carla S. Moura, Gonçalo Borrecho, António P. Alves de Matos, Joaquim M. S. Cabral, Robert J. Linhardt & Frederico Castelo Ferreira (2021) Effects of glycosaminoglycan supplementation in the chondrogenic differentiation of bone marrow- and synovial- derived mesenchymal stem/stromal cells on 3D-extruded poly (ε-caprolactone) scaffolds, International Journal of Polymeric Materials and Polymeric Biomaterials, 70:3, 207-222, DOI: 10.1080/00914037.2019.1706511pt_PT
dc.identifier.doi10.1080/00914037.2019.1706511pt_PT
dc.identifier.issn1563-535X
dc.identifier.urihttp://hdl.handle.net/10400.8/8224
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherTaylor & Francispt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectArticular cartilagept_PT
dc.subjectChondroitin sulfatept_PT
dc.subjectHyaluronic acidpt_PT
dc.subjectMesenchymal stem/stromal cellspt_PT
dc.subjectPoly (ε-caprolactone) scaffoldspt_PT
dc.titleEffects of glycosaminoglycan supplementation in the chondrogenic differentiation of bone marrow- and synovial- derived mesenchymal stem/stromal cells on 3D-extruded poly (ε-caprolactone) scaffoldspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.titleInternational Journal of Polymeric Materials and Polymeric Biomaterialspt_PT
oaire.citation.volumeVolume 70, 2021 - Issue 3pt_PT
person.familyNameMonteiro de Moura
person.givenNameCarla Sofia
person.identifier.ciencia-id671C-8FEC-A14A
person.identifier.orcid0000-0003-2610-1005
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication33d084bb-ac8d-454a-ac58-25be1331ce04
relation.isAuthorOfPublication.latestForDiscovery33d084bb-ac8d-454a-ac58-25be1331ce04

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Effects of glycosaminoglycan supplementation in the chondrogenic differentiation of bone marrow and synovial derived mesenchymal stem stromal cells.pdf
Size:
3.75 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.32 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

CDRsp - Artigos em revistas internacionais