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In situ crosslinked electrospun gelatin nanofibers for skin regeneration

2017-08-04Journal article Open access
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dc.contributor.authorDias, J. R.
dc.contributor.authorBaptista-Silva, S.
dc.contributor.authorOliveira, C. M. T. de
dc.contributor.authorSousa, A.
dc.contributor.authorOliveira, A. L.
dc.contributor.authorBártolo, P. J.
dc.contributor.authorGranja, P. L.
dc.date.accessioned2025-06-02T13:56:07Z
dc.date.available2025-06-02T13:56:07Z
dc.date.issued2017-08-04
dc.description.abstractDue to its intrinsic similarity to the extracellular matrix, gelatin electrospun nanofibrous meshes are promising scaffold structures for wound dressings and tissue engineering applications. However, gelatin is water soluble and presents poor mechanical properties, which generally constitute relevant limitations to its applicability. In this work, gelatin was in situ crosslinked with 1,4-butanediol diglycidyl ether (BDDGE) at different concentrations (2, 4 and 6 wt%) and incubation time-points (24, 48 and 72 h) at 37 °C. The physico-chemical and biological properties of BDDGE-crosslinked electrospun gelatin meshes were investigated. Results show that by changing the BDDGE concentration it is possible to produce nanofibers crosslinked in situ with well-defined morphology and modulate fiber size and mechanical properties. Crosslinked gelatin meshes show no toxicity towards fibroblasts, stimulating their adhesion, proliferation and synthesis of new extracellular matrix, thereby indicating the potential of this strategy for skin tissue engineering.eng
dc.description.sponsorshipThis study was supported by the Projects PTDC/BBB-ECT/2145/2014 and UID/Multi/04044/2013, financed by European Fonds Européen de Développement Régional (FEDER) through the Portuguese national programs Programa Operacional Factores de Competitividade (COMPETE), Portugal2020 and Norte2020, and by Portuguese funds through Fundação para a Ciência e a Tecnologia (FCT). This work is also supported by the research grants SFRH/BD/91104/2012 awarded to Juliana Dias and SFRH/BPD/90047/2012 awarded to Aureliana Sousa by FCT, and by the Investigator FCT program IF/00411/2013, awarded to Ana L. Oliveira. The authors thanks to CEMUP, University of Porto for the SEM images and, to Victoria Leiro and Marco Araújo for their support in chemical reactions interpretation
dc.identifier.citationJ.R. Dias, S. Baptista-Silva, C.M.T. de Oliveira, A. Sousa, A.L. Oliveira, P.J. Bártolo, P.L. Granja, In situ crosslinked electrospun gelatin nanofibers for skin regeneration, European Polymer Journal, Volume 95, 2017, Pages 161-173, ISSN 0014-3057, https://doi.org/10.1016/j.eurpolymj.2017.08.015
dc.identifier.doi10.1016/j.eurpolymj.2017.08.015
dc.identifier.issn0014-3057
dc.identifier.urihttp://hdl.handle.net/10400.8/13057
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier BV
dc.relationCentre for Rapid and Sustainable Product Development
dc.relationCO-ELECTROSPINNING DE NANOFIBRAS DE PCL MODIFICADO E HIDROGEL CONTENDO CÉLULAS ENCAPSULADAS PARA REGENERAÇÃO DA PELE
dc.relationBIOPRINTED CELULLARIZED HYDROGELS FOR IN SITU SKIN REGENERATION
dc.relationSERICAMED - Designing Functional Silk-Based Biomaterials for In Situ Periodontal Regeneration.
dc.relationIF/00411/2013
dc.relation.hasversionhttps://www.sciencedirect.com/journal/european-polymer-journal
dc.relation.ispartofEuropean Polymer Journal
dc.rights.uriN/A
dc.subjectIn situ crosslinking
dc.subjectElectrospun fibers
dc.subjectGelatin
dc.subjectBDDGE
dc.subjectWound dressing
dc.titleIn situ crosslinked electrospun gelatin nanofibers for skin regenerationeng
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleCentre for Rapid and Sustainable Product Development
oaire.awardTitleCO-ELECTROSPINNING DE NANOFIBRAS DE PCL MODIFICADO E HIDROGEL CONTENDO CÉLULAS ENCAPSULADAS PARA REGENERAÇÃO DA PELE
oaire.awardTitleBIOPRINTED CELULLARIZED HYDROGELS FOR IN SITU SKIN REGENERATION
oaire.awardTitleSERICAMED - Designing Functional Silk-Based Biomaterials for In Situ Periodontal Regeneration.
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FMulti%2F04044%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/FARH/SFRH%2FBD%2F91104%2F2012/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F90047%2F2012/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/Investigador FCT/IF%2F00411%2F2013%2FCP1167%2FCT0001/PT
oaire.citation.endPage176
oaire.citation.startPage161
oaire.citation.titleEuropean Polymer Journal
oaire.citation.volume95
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamFARH
oaire.fundingStreamInvestigador FCT
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
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project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
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