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The biological performance of purified supercoiled p53 plasmid DNA in different cancer cell lines

2018-10-08Journal article Open access
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dc.contributor.authorValente, J.F.A.
dc.contributor.authorSousa, A.
dc.contributor.authorGaspar, V.M.
dc.contributor.authorQueiroz, João
dc.contributor.authorSousa, F.
dc.date.accessioned2023-05-26T15:08:14Z
dc.date.available2023-05-26T15:08:14Z
dc.date.issued2018-10-08
dc.description.abstractTumor suppressor p53 remains one of the most interesting therapeutic targets in cancer gene therapy due to its consistent mutation in numerous cancers. Thus, the reinstatement of the p53 expression and function can be seen as an effective alternative for cancer treatment, motivating research in this field. In this study, L-methionine matrix was used to purify the supercoiled topoisoform of a plasmid DNA encoding the p53 protein. This pure biopharmaceutical was conjugated with liposomes to comprehensively analyze its in vitro performance and therapeutic potential in different cancer cell lines, including the lung and cervix models. A different profile of cellular responses was attained after the transfection of these cancer cell lines with the p53-pDNA. Actually, the in vitro transfection with pure sc p53-pDNA resulted in a higher expression of the tumor suppressor protein in cancer cells when compared with the native pDNA samples (oc + sc topoisoforms). Also, wild-type p53 ex pression following transfection was significantly higher in HeLa cervix cancer cells compared to that obtained in A549 lung cancer cells. Overall, our findings emphasize the potential of sc pDNA gene-based therapy, also raising awareness of the need to adjust the therapeutics, considering the feature of high heterogeneity of cancer cells.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationJ.F.A. Valente, A. Sousa, V.M. Gaspar, J.A. Queiroz, F. Sousa, The biological performance of purified supercoiled p53 plasmid DNA in different cancer cell lines, Process Biochemistry, Volume 75, 2018, Pages 240-249, ISSN 1359-5113, https://doi.org/10.1016/j.procbio.2018.09.014pt_PT
dc.identifier.doi10.1016/j.procbio.2018.09.014pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.8/8526
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S1359511318307554pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectTumor suppressor p53pt_PT
dc.subjectSupercoiled plasmid DNApt_PT
dc.subjectGene Therapypt_PT
dc.subjectL-methionine chromatographypt_PT
dc.titleThe biological performance of purified supercoiled p53 plasmid DNA in different cancer cell linespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage249pt_PT
oaire.citation.startPage240pt_PT
oaire.citation.titleProcess Biochemistrypt_PT
oaire.citation.volume75pt_PT
person.familyNameValente
person.givenNameJoana
person.identifier826126
person.identifier.ciencia-id7515-7468-212D
person.identifier.orcid0000-0002-2408-9762
person.identifier.ridM-1930-2019
person.identifier.scopus-author-id56273710100
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication7a9941e0-69b5-48f5-9068-c950fb064a4f
relation.isAuthorOfPublication.latestForDiscovery7a9941e0-69b5-48f5-9068-c950fb064a4f

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