Browsing by Author "Viana, T."
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- Choosing sheep (Ovis aries) as animal model for temporomandibular joint research: Morphological, histological and biomechanical characterization of the joint discPublication . Angelo, D.F.; Morouço, P.; Alves, N.; Viana, T.; Santos, F.; González, R.; Monje, F.; Macias, D.; Carrapiço, B.; Sousa, R.; Cavaco-Gonçalves, S.; Salvado, F.; Peleteiro, C.; Pinho, M.Preclinical trials are essential to the development of scientific technologies. Remarkable molecular and cellular research has been done using small animal models. However, significant differences exist regarding the articular behavior between these models and humans. Thus, large animal models may be more appropriate to perform trials involving the temporomandibular joint (TMJ). The aim of this work was to make a morphological (anatomic dissection and white light 3D scanning system), histological (TMJ in bloc was removed for histologic analysis) and biomechanical characterization (tension and compression tests) of sheep TMJcomparing the obtained results with human data. Results showed that sheep processus condy-laris and fossa mandibularis are anatomically similar to the same human structures. TMJ dischas an elliptical perimeter, thinner in the center than in periphery. Peripheral area actsas a ring structure supporting the central zone. The disc cells display both fibroblast andchondrocyte-like morphology. Marginal area is formed by loose connective tissue, with somechondrocyte-like cells and collagen fibers in diverse orientations. Discs obtained a tensile mod-ulus of 3.97 ± 0.73 MPa and 9.39 ± 1.67 MPa, for anteroposterior and mediolateral assessment.The TMJ discs presented a compressive modulus (E) of 446.41 ± 5.16 MPa and their maximumstress value ( max) was 18.87 ± 1.33 MPa. Obtained results suggest that these animals should beconsidered as a prime model for TMJ research and procedural training. Further investigationsin the field of oromaxillofacial surgery involving TMJ should consider sheep as a good animalmodel due to its resemblance of the same joint in humans.© 2016 Elsevier Masson SAS. All rights reserved.
- A Novel Biomanufacturing System to Produce Multi-Material Scaffolds for Tissue Engineering: Concept and Preliminary ResultsPublication . Viana, T.; Biscaia, S.; Dabrowska, E.; Franco, M.; Carreira, P.; Morouço, P.; Alves, N.This research work aims to validate a new system that enables the fabrication of multimaterial 3D structures using poly(e-caprolactone) and sodium alginate for potential use in Tissue Engineering applications. To produce multi-material scaffolds for Tissue Engineering, accurate techniques are needed to obtain three-dimensional constructs with clinically appropriate size and structural integrity. This paper presents a novel biomanufacturing system which can fabricate 3D scaffolds with precise shape and porosity, through the control of all fabrication modules by an integrated computational platform. The incorporation of a clean flow unit and a camera makes it possible to produce scaffolds in a clean environment and provides a monitoring tool to analyse constructs during the production, respectively.