Browsing by Author "Iafisco, Michele"
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- Inhalation of peptide-loaded nanoparticles improves heart failurePublication . Miragoli, Michele; Ceriotti, Paola; Iafisco, Michele; Vacchiano, Marco; Salvarani, Nicolò; Carullo, Pierluigi; Ramírez-Rodríguez, Gloria Belén; Fernandes Patrício, Tatiana Marisa; Degli Esposti, Lorenzo; Rossi, Francesca; Ravanetti, Francesca; Pinelli, Silvana; Alinovi, Rossella; Erreni, Marco; ROSSI, Stefano; Condorelli, Gianluigi; Post, Heiner; TAMPIERI, ANNA; Catalucci, DanielePeptides are highly selective and efficacious for the treatment of cardiovascular and other diseases. However, it is currently not possible to administer peptides for cardiac-targeting therapy via a noninvasive procedure, thus representing scientific and technological challenges. We demonstrate that inhalation of small (<50 nm in diameter) biocompatible and biodegradable calcium phosphate nanoparticles (CaPs) allows for rapid translocation of CaPs from the pulmonary tree to the bloodstream and to the myocardium, where their cargo is quickly released. Treatment of a rodent model of diabetic cardiomyopathy by inhalation of CaPs loaded with a therapeutic mimetic peptide that we previously demonstrated to improve myocardial contraction resulted in restoration of cardiac function. Translation to a porcine large animal model provides evidence that inhalation of a peptide-loaded CaP formulation is an effective method of targeted administration to the heart. Together, these results demonstrate that inhalation of biocompatible tailored peptide nanocarriers represents a pioneering approach for the pharmacological treatment of heart failure.
- Superparamagnetic hybrid microspheres affecting osteoblasts behaviourPublication . Fernandes Patrício, Tatiana Marisa; Panseri, Silvia; Montesi, Monica; Iafisco, Michele; Sandri, Monica; Tampieri, Anna; Sprio, SimoneThe present work describes biomimetic hybrid microspheres made of collagen type I-like peptide matrix (RCP) mineralised with Fe2+/Fe3+ doping hydroxyapatite (RCPFeHA) by a bio-inspired process. Superparamagnetic RCPFeHA microspheres are obtained by emulsification of the hybrid slurries in the presence of citrate ions, to achieve a biomimetic surface functionalisation improving the bioactivity and the dispersion ability in cell culture medium. A biological in vitro study correlates the osteoblast cells behaviour to calcium and iron ions released by the hybrid microspheres in culture media mimicking physiological or inflammatory environment, evidencing a clear triggering of cell activity and bio-resorption ability. In presence of the microspheres, the osteoblast cells maintain their typical morphology and no cell damage were detected, whereas also showing up-regulation of osteogenic markers. The ability of the hybrid microspheres to undergo bio-resorption and release bioactive ions in response to different environmental stimuli without harmful effects opens new perspectives in bone regeneration, as magnetically active bone substitute with potential ability of drug carrier and smart response in the presence of inflammatory states.