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Produção de lapas da espécie Patella depressa (Pennant, 1777) em cativeiro
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A reprodução e cultivo larvar de Patella depressa em laboratório é limitada pelo conhecimento insuficiente desta espécie. Visando otimizar o protocolo experimental, foram realizados pré-ensaios para determinar o método de maturação de oócitos mais eficiente (água salgada ou banho alcalino com NHHOH), tipo de água mais indicada (Furo ou Docapesca), intervalo ideal entre o início da fecundação e avaliação da respetiva taxa (3 e 4 horas) e temperatura de incubação ideal (16 e 18°C). Os resultados obtidos nos pré-ensaios permitiram definir banho alcalino com NHHOH como método mais eficiente de maturação de oócitos, água da Docapesca como mais adequada à
reprodução, e ainda que a taxa de fertilização deve ser calculada 3 horas pós fertilização
e que a reprodução deve ocorrer a 16°C. Nos ensaios principais, avaliou-se a concentração de oócitos inicial (100 e 200 oócitos/ml) e regime de alimentação (Sem Alimentação e Com Alimentação) sobre a taxa de fertilização, eclosão e sobrevivência larvar. Adicionalmente, avaliou-se o potencial de utilizar biofilmes de Phaeodactylum tricornutum, Tetraselmis suecica e biofilme resultante de tanques de cultivo de pepinos do mar como substratos de assentamento. Com os resultados obtidos foi possível determinar que a concentração de oócitos inicial com melhores resultados foi 100 oócitos/ml e que a alimentação na fase inicial de desenvolvimento de larvas mostrou ser desnecessária. Nenhum dos biofilmes escolhidos promoveu o assentamento larvar.
Estes resultados fornecem informações fundamentais ao desenvolvimento de protocolos de reprodução de P. depressa, mas deixam ainda algumas lacunas por preencher.
The reproduction and larval development of Patella depressa larvae in the laboratory conditions is limited due to insufficient knowledge of this species. To optimize the experimental protocol, pre-tests were conducted to determine the most efficient method for oocyte maturation (salt water or an alkaline bath with NHHOH), the most suitable water type (Furo or Docapesca), the ideal interval between the start of fertilization and the evaluation of the respective rate (3 and 4 hours) and the ideal incubation temperature (16 and 18°C). In the main trials, the effect of initial oocyte concentrations (100 and 200 oocytes/ml) and feeding regime (Without Feeding and With Feeding) were evaluated on the rate of fertilization, hatching and larval survival. The potential of using Phaeodactylum tricornutum and Tetraselmis suecica biofilms, and biofilms resulting from sea cucumber culture tanks as settlement substrates was also evaluated. The results obtained made it possible to determine that the initial oocyte concentration with the best suited for reproduction of P. depressa was 100 oocytes/ml, and feeding during the early larval development phase was shown to be unnecessary. None of the selected biofilms promoted larval settlement. These results provide fundamental information for the development of P. depressa breeding protocols but leave gaps in knowledge to be filled.
The reproduction and larval development of Patella depressa larvae in the laboratory conditions is limited due to insufficient knowledge of this species. To optimize the experimental protocol, pre-tests were conducted to determine the most efficient method for oocyte maturation (salt water or an alkaline bath with NHHOH), the most suitable water type (Furo or Docapesca), the ideal interval between the start of fertilization and the evaluation of the respective rate (3 and 4 hours) and the ideal incubation temperature (16 and 18°C). In the main trials, the effect of initial oocyte concentrations (100 and 200 oocytes/ml) and feeding regime (Without Feeding and With Feeding) were evaluated on the rate of fertilization, hatching and larval survival. The potential of using Phaeodactylum tricornutum and Tetraselmis suecica biofilms, and biofilms resulting from sea cucumber culture tanks as settlement substrates was also evaluated. The results obtained made it possible to determine that the initial oocyte concentration with the best suited for reproduction of P. depressa was 100 oocytes/ml, and feeding during the early larval development phase was shown to be unnecessary. None of the selected biofilms promoted larval settlement. These results provide fundamental information for the development of P. depressa breeding protocols but leave gaps in knowledge to be filled.
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Assentamento Cultivo Larvar Lapas Patella depressa Reprodução