Vieira, Paula Cristina Rodrigues Pascoal FariaAscenso, Rita Margarida TeixeiraMoura, Carla Sofia Monteiro deFernandes, Cristiana Henriques2020-01-162020-01-162019-10-24http://hdl.handle.net/10400.8/4440Over the last decades, the number of occupational accidents associated with bone problems has increased leading to a growing concern worldwide. Currently, autografts, allografts, and xenografts are used for bone regeneration. However, their application have associated risks. Tissue Engineering (TE) has investigated solutions to address these problems, specifically through the production of temporary supports, also called biocompatible and biodegradable scaffolds, which allow cell adhesion and proliferation, providing mechanical support to the formation of new bone tissue. These three-dimensional supports must meet certain requirements, namely mechanical and biological. Upon its production and in order to give an appropriate answer, the material must be biocompatible as well as the products derived from its degradation. The combination of materials and structure with the technique to be used will directly influence their physical and chemical properties and, consequently, their action in contributing to bone regeneration. Thus, the focus of this dissertation is to perform a critical analysis of the state-of-art in bone TE and present a proposal of an optimized temporary support geometry. For this, the choice of biomaterials, cells and their culture conditions (static vs. dynamic) and/or the addition of growth factors for the repair of large bone defects are addressed is addressed.engBioreactorBone regeneration CADScaffoldsTissue engineeringmaster thesis202368416