Moura, Carla SofiaSilva, Cláudia Lobato daBartolo, Paulo JorgeFerreira, Frederico Castelo2025-12-152025-12-152015Monteiro de Moura, Carla Sofia & Silva, Cláudia & Bártolo, Paulo & Ferreira, Frederico. (2015). Combination of 3D Extruded-based Poly (ɛ-caprolactone) Scaffolds with Mesenchymal Stem/Stromal Cells: Strategy Optimization. Procedia Engineering. 110. 122-127. 10.1016/j.proeng.2015.07.019.1877-7058http://hdl.handle.net/10400.8/15035Conference name 4th International Conference on Tissue Engineering - An ECCOMAS Thematic Conference, ICTE 2015, LisbonConference date 25 June 2015 - 27 June 2015Part of special issue 4th International Conference on Tissue Engineering, ICTE2015, An ECCOMAS Thematic ConferenceThis study uses a combination of 3D extruded-based biodegradable scaffolds and human bone marrow-derived mesenchymal stem/stromal cells to understand how scaffold properties can contribute for efficient cell seeding, proliferation and migration. Highly interconnected porous poly(ɛ-caprolactone) scaffolds were produced by a layer-by-layer extrusion. Two configurations were studied with larger (390 μm) or smaller pore size (190 μm) at the centre. MSC, seeded on the top centre of the scaffold surface, increased 5 folds after 13 days of cultivation for both configurations, allowing us to conclude that in this range of pore sizes pose no limitations for cell migration are observed.engMesenchymal stem/stromal cellsPoly (ε-caprolactone)ScaffoldsTissue engineeringjournal article10.1016/j.proeng.2015.07.019