Lopes, João M.Marques-da-Silva, DorindaVideira, Paula A.Lagoa, Ricardo2026-03-162026-03-162022-06-29Lopes, J. M., Marques-da-Silva, D., Videira, P. A., & Lagoa, R. (2022). Use of Lipid Vesicles for Revealing the Potential Contribution of Cytochrome C in the Metabolism of Environmental Toxicants. Medical Sciences Forum, 11(1), 6. https://doi.org/10.3390/BiTaP-12782http://hdl.handle.net/10400.8/15879Article number - 6This article belongs to the Proceedings of Biosystems in Toxicology and Pharmacology — Current Challenges.Presented at the Biosystems in Toxicology and Pharmacology—Current Challenges, 8–9 September 2022, Online; Available online: https://bitap.sciforum.net/.Cytochrome c (Cc) is well-known as an electron carrier at mitochondria but can also catalyze peroxidase-like reactions. Although the reported catalytic abilities are low, the role of Cc in transforming environmental toxicants requires additional studies. We employed cardiolipin vesicles to stimulate the enzymatic activity of Cc and tested the catalytic system towards the dye methyl orange and the polycyclic aromatic hydrocarbons (PAHs) benzo[b]fluoranthene and benzo[a]pyrene. The results showed that cardiolipin-containing vesicles promoted dye decolorization and the oxidation of both PAHs by Cc, indicating that lipid membranes can be useful to further investigate Cc–mediated metabolism of toxicants.engazo dyepseudo-peroxidasephospholipidenvironmental toxicologybiocatalytic systemsconference paper10.3390/bitap-127822673-9992