Cabral, C.Poças Almeida, JulianaGonçalves, M.J.Cavaleiro, C.Cruz, M.T.Salgueiro, L.2025-07-072025-07-072015-03Cabral, Célia & Poças, Juliana & Gonçalves, Maria & Cavaleiro, Carlos & Salgueiro, Ligia. (2015). Ridolfia segetum (L.) Moris (Apiaceae) from Portugal: A source of safe antioxidant and anti-inflammatory essential oil. Industrial Crops and Products. 65. 10.1016/j.indcrop.2014.11.041.0926-6690http://hdl.handle.net/10400.8/13562Ridolfia segetum (L.) Moris essential oil was obtained by hydrodistillation and analysed by GC and GC-MS. The antioxidant properties were evaluated through the TBARS assay, and the in vitro anti-inflammatory activity was assessed through inhibition of NO production triggered by the Toll-like receptor 4 (TLR4) agonist, lipopolysaccharide (LPS), in mouse macrophages. Assessment of cell viability was made through a colorimetric assay using MTT on macrophages, keratinocytes, and hepatocytes. The main compounds are α-phellandrene (53.0-63.3%), terpinolene (11.9-8.6%), ß-phellandrene (5.5-6.0%), and dillapiol (1.9-8.0%). The oil demonstrated high antioxidant capacity in the concentrations tested (0.05-0.20. μL/mL) and significantly inhibited NO production without cytotoxicity in concentrations up to 1.25. μL/mL. The plants showed a high essential oil yield, which confers an industrial potential interest. Also, the high content of α-phellandrene (53.0-63.3%) makes it an important natural source of this compound. These results provide evidence of the therapeutic effects of this oil, and suggest that the anti-inflammatory activity in doses without cytotoxicity on mammalian cells, combined with its antioxidant potential and pleasant smell, could be of great value in the development of new phytopharmaceuticals.engAnti-inflammatory activityAntioxidant activityCytotoxicityEssential oilRidolfia segetumα-Phellandrenejournal article10.1016/j.indcrop.2014.11.041