Lopes, GracilianaSousa, CarlaBernardo, JoãoAndrade, Paula B.Valentão, PatríciaFerreres, FedericoMouga, Teresa2023-03-162023-03-162011Lopes, G., Sousa, C., Bernardo, J., Andrade, P.B., Valentão, P., Ferreres, F. and Mouga, T. (2011), STEROL PROFILES IN 18 MACROALGAE OF THE PORTUGUESE COAST. Journal of Phycology, 47: 1210-1218. https://doi.org/10.1111/j.1529-8817.2011.01028.x1529-88170022-3646http://hdl.handle.net/10400.8/8241The sterol profiles of dominant macroalgae occur-ring in the western Portuguese coast were evaluated.An analytical procedure, involving alkaline hydrolysisand extraction followed by separation by reversed-phase HPLC–diode array detection (HPLC–DAD),was optimized for the study of their sterols composi-tion. The validated methodology is short in analysistime (as the compounds are determined in <20 min),sensitive, reproducible, and accurate. It was thensuccessfully applied to the determination of campes-terol, cholesterol, desmosterol, ergosterol, fucosterol,stigmasterol, andb-sitosterol in 18 species (threeChlorophyta, five Rhodophyta, and 10 Phaeophyta).The profiles obtained for the several macroalgalspecies were considerably different. C29 sterolswere predominant in Phaeophyta and Chlorophyta(71%–95% of total sterol content), while in Rhodo-phyta cholesterol content is significantly higher(34%–87%). Among the studied species,AsparagopsisarmataHarv. contained the lowest sterol amount(555 mgÆkg)1dry weight), andCystoseira tamariscifo-lia(Huds.) Papenf. the highest one (6,502 mgÆkg)1dry weight). Data obtained may be helpful inidentifying suitable marine sources of sterols, withpotential applications in the food and pharmaceutical industries.engChlorophytaMacroalgaePha-eophytaRhodophytaSterolsjournal article10.1111/j.1529-8817.2011.01028.x