Silva, ZéliaAlmeida, DanielleMarques-da-Silva, DorindaLagoa, RicardoVideira, Paula A.2026-03-232026-03-232022-07-13Silva, Z., Almeida, D., Marques-da-Silva, D., Lagoa, R., & Videira, P. A. (2022). Oxidative Stress and Inflammatory Response of Skin Fibroblasts Exposed to Chlorpyrifos. Medical Sciences Forum, 11(1), 7. https://doi.org/10.3390/BiTaP-12791http://hdl.handle.net/10400.8/15943Article number - 7This article belongs to the Proceedings Biosystems in Toxicology and Pharmacology—Current Challenges.All authors have read and agreed to the published version of the manuscript.Medical Sciences Forum, Volume 11, Issue 1 BiTaP 2022 Biosystems in Toxicology and Pharmacology—Current Challenges Online | 8–9 September 2022Chlorpyrifos (CPF) is a widely used insecticide. The aim of this work was to study the effect of CPF in skin fibroblasts exposed to concentrations detected in human skin and unleash underlying cellular mechanisms. Fibroblasts were exposed to different concentrations (0.36–250 µM) of CPF pure alone or in a commercial CPF mixture (Lethal 20) for 6 days. In conclusion, prolonged exposure to 250 µM of CPF pure and 125 µM of Lethal 20 caused a significant loss of the fibroblast´s viability. Moreover, the toxicity of this pesticide in fibroblasts is evidenced by the induction of oxidative stress and stimulation of the production of interleukin (IL)-6.engchlorpyrifosimmunotoxicologyinflammationoxidative stressskin fibroblastsIL-6conference paper10.3390/bitap-127912673-9992