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Title: Enzymatic depolymerisation of cellulose
Authors: Pala, Helena
Mota, Manuel
Gama, Francisco Miguel
Keywords: Cellulase
Degree of polimerisation
Gel permeation chromatography
Paper pulps
Issue Date: Mar-2007
Publisher: Elsevier
Citation: PALA, Helena; MOTA, Manuel; GAMA, Francisco Miguel - Enzymatic depolymerisation of cellulose. Carbohydrate Polymers. Vol. 68, n.º 1 (Mar. 2007), p. 101-108.
Abstract: Cellulose degree of polymerisation (DP) was determined in paper pulps and commercial powder celluloses before and after the enzymatic treatment with a blend of cellulases and hemicellulases. Three different methods were used: (i) chemical method, based on the measurement of the cellulose reducing power; (ii) viscosimetry; and (iii) gel permeation chromatography. Although based in very different principles, the three methods find similar DP values in the case of pure cellulose with narrow molecular weight distributions. The chemical method is more sensitive to detect enzymatic depolymerisation and appears to provide a good estimation of the number-average DP. GPC data offers more detailed information about the enzymes mode of action on the fibres, showing that cellulose hydrolysis in the presence of the commercial preparation Celluclast 1.5L follows a layer-by-layer solubilisation mechanism. 2006 Elsevier Ltd. All rights reserved.
ISSN: 0144-8617
Appears in Collections:Artigos em revistas internacionais

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