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|Título: ||Enzymatic depolymerisation of cellulose|
|Autor: ||Pala, Helena|
Gama, Francisco Miguel
Degree of polimerisation
Gel permeation chromatography
|Issue Date: ||Mar-2007|
|Citação: ||PALA, Helena; MOTA, Manuel; GAMA, Francisco Miguel - Enzymatic depolymerisation of cellulose. Carbohydrate Polymers. Vol. 68, n.º 1 (Mar. 2007), p. 101-108.|
|Resumo: ||Cellulose degree of polymerisation (DP) was determined in paper pulps and commercial powder celluloses before and after the enzymatic
treatment with a blend of cellulases and hemicellulases. Three different methods were used: (i) chemical method, based on the measurement
of the cellulose reducing power; (ii) viscosimetry; and (iii) gel permeation chromatography. Although based in very different
principles, the three methods find similar DP values in the case of pure cellulose with narrow molecular weight distributions. The chemical
method is more sensitive to detect enzymatic depolymerisation and appears to provide a good estimation of the number-average DP.
GPC data offers more detailed information about the enzymes mode of action on the fibres, showing that cellulose hydrolysis in the presence
of the commercial preparation Celluclast 1.5L follows a layer-by-layer solubilisation mechanism.
2006 Elsevier Ltd. All rights reserved.|
|Appears in Collections:||Artigos em revistas internacionais|
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